Journal
METABOLITES
Volume 13, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/metabo13050602
Keywords
tryptophan; indolepropionic acid; indolelactic acid; indoleacetic acid; indoxylsulfate; indole; plasma ultrafiltrate; saliva; gut metabolome
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In this study, the microbial metabolite indolepropionic acid (IPA) and related indolic metabolites were measured in human plasma, plasma ultrafiltrate, and saliva. The levels of IPA in plasma ultrafiltrate and of indolelactic acid (ILA) in saliva were reported for the first time. The detection of free plasma IPA provides valuable information on the physiologically active pool of this important microbial metabolite.
The microbial metabolite indolepropionic acid (IPA) and related indolic metabolites, including indolecarboxylic acid (ICA), indolelactic acid (ILA), indoleacetic acid (IAA), indolebutyric acid (IBA), indoxylsulfate (ISO4), and indole, were determined in human plasma, plasma ultrafiltrate (UF), and saliva. The compounds were separated on a 150 x 3 mm column of 3 mu m Hypersil C18 eluted with a mobile phase of 80% pH 5 0.01 M sodium acetate containing 1.0 g/L of tert-butylammonium chloride/20% acetonitrile and then detected fluorometrically. Levels of IPA in human plasma UF and of ILA in saliva are reported for the first time. The determination of IPA in plasma UF enables the first report of free plasma IPA, the presumed physiologically active pool of this important microbial metabolite of tryptophan. Plasma and salivary ICA and IBA were not detected, consistent with the absence of any prior reported values. Observed levels or limits of detection for other indolic metabolites usefully supplement limited prior reports.
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