Journal
FRONTIERS IN MOLECULAR BIOSCIENCES
Volume 10, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmolb.2023.1211621
Keywords
tannase; pH stability; tannin biodegradation; gallate esters; gallic acid
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In this study, the tannin-degrading strain Debaryomyces hansenii was screened and the tannase coding gene TANF was successfully cloned and expressed in Yarrowia lipolytica. The purified TanF tannase showed optimal activity at 40 degrees C and retained over 80% of its activity in the range of 35 degrees C-60 degrees C. It exhibited remarkable enzyme activity at pH 5.0 and retained more than 70% of its relative activity across a wide pH range of 3.0-8.0. TanF also demonstrated broad substrate specificity for gallate esters and achieved high gallic acid production from tannic acid.
Introduction: Tannase is a crucial enzyme that finds wide applications in the pharmaceutical industry, feed processing, and beverage manufacturing. Although extensive studies have been conducted on tannases from fungi and bacteria, reports on tannases exhibiting favorable pH stability are relatively limited. Methods: In this study, a tannin-degrading strain Debaryomyces hansenii was screened to induce tannase production, and the corresponding tannase coding gene TANF was successfully cloned and expressed in Yarrowia lipolytica. SDS-PAGE analysis revealed that the purified TanF tannase had a molecular weight of approximately 70 kDa.Results and Discussion: The enzyme demonstrated optimal activity at 40 degrees C and retained over 80% of its activity in the range of 35 degrees C-60 degrees C. Of particular interest, TanF exhibited remarkable enzyme activity at pH 5.0 and retained more than 70% of its relative activity across a wide pH range of 3.0-8.0. Furthermore, TanF exhibited broad substrate specificity for gallate esters. The final gallic acid production by TanF from tannic acid achieved 18.32 g/L. Therefore, the excellent properties TanF has been demonstrated to be an efficient tool for the preparation of gallic acid.
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