4.6 Article

A Streamlined Approach for Fluorescence Labelling of Low-Copy-Number Plasmids for Determination of Conjugation Frequency by Flow Cytometry

Related references

Note: Only part of the references are listed.
Article Genetics & Heredity

Estimating plasmid conjugation rates: A new computational tool and a critical comparison of methods

Jana S. Huisman et al.

Summary: This study compares commonly used methods for measuring plasmid conjugation rates and finds that these estimates are often dependent on factors such as measurement time, population densities, and initial ratios of donor to recipient populations. The study proposes a new method to estimate conjugation rates and provides analytical expressions for the valid parameter range. In addition, a user-friendly software package and web interface are provided for accurate and comparable measurement of plasmid conjugation rates.

PLASMID (2022)

Article Ecology

Plasmid- and strain-specific factors drive variation in ESBL-plasmid spread in vitro and in vivo

Fabienne Benz et al.

Summary: This study found that clinical Escherichia coli strains associated with ESBL-producing plasmids could conjugate with other E. coli and Salmonella strains in the absence of antibiotics, with transconjugant frequencies influenced by plasmid properties, donor and recipient strains, as well as the presence of transfer genes on ESBL-plasmids and plasmid incompatibility. The results suggest that plasmid spread in the complex gut environment of animals and humans can be predicted based on in vitro testing and genetic data.

ISME JOURNAL (2021)

Article Biochemical Research Methods

Practical Guidelines for Optimization and Characterization of the Beckman Coulter CytoFLEX™ Platform

Debajit Bhowmick et al.

CYTOMETRY PART A (2020)

Article Biology

Robust estimation of bacterial cell count from optical density

Jacob Beal et al.

COMMUNICATIONS BIOLOGY (2020)

Article Multidisciplinary Sciences

Salmonella persisters promote the spread of antibiotic resistance plasmids in the gut

Erik Bakkeren et al.

NATURE (2019)

Review Microbiology

Mobile Genetic Elements Associated with Antimicrobial Resistance

Sally R. Partridge et al.

CLINICAL MICROBIOLOGY REVIEWS (2018)

Article Microbiology

Predictive Modeling of a Batch Filter Mating Process

Akshay Malwade et al.

FRONTIERS IN MICROBIOLOGY (2017)

Article Multidisciplinary Sciences

Plasmid interference for curing antibiotic resistance plasmids in vivo

Muhammad Kamruzzaman et al.

PLOS ONE (2017)

Review Chemistry, Multidisciplinary

Applications and challenges for single-bacteria analysis by flow cytometry

Lina Wu et al.

SCIENCE CHINA-CHEMISTRY (2016)

Article Biochemical Research Methods

Rapid and Efficient One-Step Metabolic Pathway Integration in E-coli

Marcelo C. Bassalo et al.

ACS SYNTHETIC BIOLOGY (2016)

Article Engineering, Biomedical

Controlling E. coli Gene Expression Noise

Kyung Hyuk Kim et al.

IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS (2015)

Review Dermatology

EDTA: An Antimicrobial and Antibiofilm Agent for Use in Wound Care

Simon Finnegan et al.

ADVANCES IN WOUND CARE (2015)

Review Biochemistry & Molecular Biology

Survival Guide: Escherichia coli in the Stationary Phase

P. Pletnev et al.

ACTA NATURAE (2015)

Article Infectious Diseases

Antibiotic Resistance: From Darwin to Lederberg to Keynes

Carlos F. Amabile-Cuevas

MICROBIAL DRUG RESISTANCE (2013)

Article Microbiology

Genome Sequence of Escherichia coli J53, a Reference Strain for Genetic Studies

Hana Yi et al.

JOURNAL OF BACTERIOLOGY (2012)

Article Genetics & Heredity

Determination of conjugation rates on solid surfaces

Irene del Campo et al.

PLASMID (2012)

Review Biology

Conjugative plasmids: vessels of the communal gene pool

Anders Norman et al.

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES (2009)

Article Microbiology

blaIMP-4 in different genetic contexts in Enterobacterzaceae isolates from Australia

Bjoern A. Espedido et al.

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY (2008)

Article Microbiology

Cell division in Escherichia coli cultures monitored at single cell resolution

Johanna Roostalu et al.

BMC MICROBIOLOGY (2008)

Article Biochemistry & Molecular Biology

Conjugation in Escherichia coli -: A laboratory exercise

Somkiat Phornphisutthimas et al.

BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION (2007)

Article Biotechnology & Applied Microbiology

Induction of a global stress response during the first step of Escherichia coli plate growth

Caroline Cuny et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2007)

Review Microbiology

Mechanisms of, and barriers to, horizontal gene transfer between bacteria

CM Thomas et al.

NATURE REVIEWS MICROBIOLOGY (2005)

Article Biochemical Research Methods

High frequency transformation of the amphotericin-producing bacterium Streptomyces nodosus

J Nikodinovic et al.

JOURNAL OF MICROBIOLOGICAL METHODS (2003)

Article Biochemical Research Methods

Use of SYTOX green dye in the flow cytometric analysis of bacterial phagocytosis

JJ Gaforio et al.

CYTOMETRY (2002)

Review Multidisciplinary Sciences

Lateral gene transfer and the nature of bacterial innovation

H Ochman et al.

NATURE (2000)