Detection of Oxacillin/Cefoxitin Resistance in Staphylococcus aureus Present in Recurrent Tonsillitis

Background: Recurrent tonsillitis is one of the most common diseases in childhood, caused many times by ss-lactam-resistant S. aureus. The objective of this study was to investigate an alternative method to identify resistance to oxacillin/cefoxitin in S. aureus from hospitalized children with recurrent tonsillitis. Methods: The samples of S. aureus came from patients with recurrent tonsillitis and were used in 16S rRNA sequencing and an antibiogram test for identification and verifying resistance, after which HSI methodology were applied for separation of S. aureus resistances. Results: The S. aureus isolated showed sensitivity to oxacillin/cefoxitin and the diagnostic images show a visual description of the resistance different groups formed, that may be related to sensitivity and resistance to oxacillin/cefoxitin, characterizing the MRSA S. aureus. Conclusions: Samples that showed phenotypic resistance to oxacillin/cefoxitin were clearly separated from samples that did not show this resistance. A PLS-DA model predicted the presence of resistance to oxacillin/cefoxitin in S. aureus samples and it was possible to observe the pixels classified as MRSA. The HSI was able to successfully discriminate samples in replicas that were sensitive and resistant, based on the calibration model it received.

Automated summary

This study aimed to explore an alternative method for identifying resistance to oxacillin/cefoxitin in S. aureus from hospitalized children with recurrent tonsillitis. Through 16S rRNA sequencing and antibiogram testing, a feasible separation of resistance based on HSI methodology was obtained, and the MRSA S. aureus characteristics were found. The samples that showed phenotypic resistance to oxacillin/cefoxitin were clearly separated from those that did not, and the PLS-DA model successfully predicted the presence of resistance.