Whole Genome Sequencing of the Novel Probiotic Strain Lactiplantibacillus plantarum FCa3L

Lactiplantibacillus plantarum is best known for its significant adaptive potential and ability to colonize different ecological niches. Different strains of L. plantarum are widely used as probiotics. To characterize the probiotic potential of the novel L. plantarum FCa3L strain isolated from fermented cabbage, we sequenced its whole genome using the Illumina MiSeq platform. This bacterial isolate had a circular chromosome of 3,365,929 bp with 44.3% GC content and a cyclic phage phiX174 of 5386 bp with 44.7% GC content. The results of in vitro studies showed that FCa3L was comparable with the reference probiotic strain L. plantarum 8PA3 in terms of acid and bile tolerance, adhesiveness, H2O2 production, and acidification rate. The strain 8PA3 possessed higher antioxidant activity, while FCa3L demonstrated superior antibacterial properties. The antibiotic resistance of FCa3L was more relevant to the probiotic strain than that of 8PA3, although a number of silent antibiotic resistance genes were identified in its genome. Genomic evidence to support adhesive and antibacterial properties, biosynthesis of bioactive metabolites, and safety of FCa3L was also presented. Thus, this study confirmed the safety and probiotic properties of L. plantarum FCa3L via complete genome and phenotype analysis, suggesting its potential as a probiotic, although further in vivo investigations are still necessary.

Automated summary

Lactiplantibacillus plantarum is a versatile bacterium widely used as a probiotic. In this study, the probiotic potential of a novel strain called L. plantarum FCa3L, isolated from fermented cabbage, was characterized through genome sequencing. The results showed that FCa3L had comparable properties to the reference strain, L. plantarum 8PA3, in terms of acid and bile tolerance, adhesiveness, H2O2 production, and acidification rate. FCa3L also exhibited superior antibacterial properties, although it had a higher antibiotic resistance gene content. The study provided genomic evidence supporting the adhesive and antibacterial properties, biosynthesis of bioactive metabolites, and safety of FCa3L, suggesting its potential as a probiotic.