Journal
MICROORGANISMS
Volume 11, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/microorganisms11030604
Keywords
chlamydial infection; IL-27; dendritic cells; maturation; Th1 response; IL-10
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IL-27/IL-27R signaling plays a protective role in Chlamydia trachomatis infection by promoting Th1 response and suppressing neutrophilic inflammation. IL-27 expression was observed in dendritic cells (DCs), and its signaling in DCs restricted the production of IL-10, thus supporting the Th1 response against chlamydial infection.
Chlamydia trachomatis usually causes mucosal infections, bringing considerable morbidity and socioeconomic burden worldwide. We previously revealed that IL-27/IL-27R mediates protection against chlamydial invasion by promoting a protective Th1 response and suppressing neutrophilic inflammation. Here, we used the mouse model of Chlamydia muridarum (C. muridarum) respiratory infections to further investigate the impact of IL-27 signaling in the DCs-regulated immune response, since an elevated IL-27/IL-27R expression in DCs was identified following chlamydial infection. An adoptive transfer of Chlamydia muridarum-stimulated DCs to wild-type mice approach was subsequently used, and the donor-DCs-promoted resistance with a higher Th1 response against chlamydial infection was attenuated when DCs lacking IL-27R were used as donor cells. Flow cytometry analysis revealed the suppression of IL-27 signaling on DCs phenotypic maturation. A further functional maturation analysis of DCs revealed that IL-27 signaling restricted the protein and mRNA expression of IL-10 from DCs following infection. Thus, these findings suggest that IL-27 signaling could support the Th1 response via inhibiting IL-10 production in DCs, thus mediating the protective host defense against chlamydial respiratory infection.
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