Antibiotic-Resistant Desulfovibrio Produces H2S from Supplements for Animal Farming

Sulphate-reducing bacteria, primarily Desulfovibrio, are responsible for the active generation of H2S in swine production waste. The model species for sulphate reduction studies, Desulfovibrio vulgaris strain L2, was previously isolated from swine manure characterized by high rates of dissimilatory sulphate reduction. The source of electron acceptors in low-sulphate swine waste for the high rate of H2S formation remains uncertain. Here, we demonstrate the ability of the L2 strain to use common animal farming supplements including L-lysine-sulphate, gypsum and gypsum plasterboards as electron acceptors for H2S production. Genome sequencing of strain L2 revealed the presence of two megaplasmids and predicted resistance to various antimicrobials and mercury, which was confirmed in physiological experiments. Most of antibiotic resistance genes (ARG) are carried by two class 1 integrons located on the chromosome and on the plasmid pDsulf-L2-2. These ARGs, predicted to confer resistance to beta-lactams, aminoglycosides, lincosamides, sulphonamides, chloramphenicol and tetracycline, were probably laterally acquired from various Gammaproteobacteria and Firmicutes. Resistance to mercury is likely enabled by two mer operons also located on the chromosome and on pDsulf-L2-2 and acquired via horizontal gene transfer. The second megaplasmid, pDsulf-L2-1, encoded nitrogenase, catalase and type III secretion system suggesting close contact of the strain with intestinal cells in the swine gut. The location of ARGs on mobile elements allows us to consider D. vulgaris strain L2 as a possible vector transferring antimicrobials resistance determinants between the gut microbiote and microbial communities in environmental biotopes.

Automated summary

Sulphate-reducing bacteria, particularly Desulfovibrio, play a key role in the production of H2S in swine waste. Desulfovibrio vulgaris strain L2, a model organism for sulphate reduction studies, can utilize animal farming supplements as electron acceptors for H2S production. The genome sequencing of strain L2 revealed the presence of antibiotic resistance genes (ARG) and genes conferring resistance to mercury. These resistance genes are likely acquired through horizontal gene transfer from other bacteria. The presence of ARGs on mobile genetic elements suggests strain L2 may contribute to the transfer of antimicrobial resistance determinants between the gut microbiota and environmental microbial communities.