Journal
ANTIOXIDANTS
Volume 12, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/antiox12061207
Keywords
rheumatoid arthritis; pain; cartilage; chondrocyte; interleukin-1 beta; inflammation; oxidative stress; VEGF-A; disease modifying agent; pharmacological modulator
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Rheumatoid arthritis is a chronic autoimmune disorder characterized by joint pain, swelling, and movement impairment caused by inflammation-induced cartilage and bone degradation. Recent research has identified FPRs as a potential target for treatment, and AMC3, a novel agonist, has shown efficacy in vitro and in vivo. This study demonstrates the usefulness of AMC3 and lays the foundation for further research.
Rheumatoid arthritis is an autoimmune disorder that causes chronic joint pain, swelling, and movement impairment, resulting from prolonged inflammation-induced cartilage and bone degradation. The pathogenesis of RA, which is still unclear, makes diagnosis and treatment difficult and calls for new therapeutic strategies to cure the disease. Recent research has identified FPRs as a promising druggable target, with AMC3, a novel agonist, showing preclinical efficacy in vitro and in vivo. In vitro, AMC3 (1-30 mu M) exhibited significant antioxidant effects in IL-1 beta (10 ng/mL)-treated chondrocytes for 24 h. AMC3 displayed a protective effect by downregulating the mRNA expression of several pro-inflammatory and pro-algic genes (iNOS, COX-2, and VEGF-A), while upregulating genes essential for structural integrity (MMP-13, ADAMTS-4, and COLIAI). In vivo, AMC3 (10 mg kg(-1)) prevented hypersensitivity and restored postural balance in CFA-injected rats after 14 days. AMC3 attenuated joint alterations, reduced joint inflammatory infiltrate, pannus formation, and cartilage erosion. Chronic AMC3 administration reduced transcriptional changes of genes causing excitotoxicity and pain (EAATs and CCL2) and prevented morphological changes in astrocytes, including cell body hypertrophy, processes length, and thickness, caused by CFA in the spinal cord. This study demonstrates the usefulness of AMC3 and establishes the groundwork for further research.
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