Journal
BIOMOLECULES
Volume 13, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/biom13040654
Keywords
alternative splicing; exon-inclusion; exon-skipping; group I intron; ribozyme; self-splicing; Tetrahymena
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Alternative splicing is an important mechanism in eukaryotic cells that allows multiple proteins to be produced from a single gene. Although it is usually associated with group I self-splicing introns, limited examples of alternative splicing have been reported. This study focuses on exon-skipping splicing in genes containing two group I introns and reveals the key structural elements important for this type of splicing through pairwise engineering and biochemical characterization.
Alternative splicing is an important mechanism in the process of eukaryotic nuclear mRNA precursors producing multiple protein products from a single gene. Although group I self-splicing introns usually perform regular splicing, limited examples of alternative splicing have also been reported. The exon-skipping type of splicing has been observed in genes containing two group I introns. To characterize splicing patterns (exon-skipping/exon-inclusion) of tandemly aligned group I introns, we constructed a reporter gene containing two Tetrahymena introns flanking a short exon. To control splicing patterns, we engineered the two introns in a pairwise manner to design pairs of introns that selectively perform either exon-skipping or exon-inclusion splicing. Through pairwise engineering and biochemical characterization, the structural elements important for the induction of exon-skipping splicing were elucidated.
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