Journal
VACCINES
Volume 11, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/vaccines11061053
Keywords
norovirus; recombinant P protein; neutralizing antibody; immunogenicity; blocking efficacy
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This study aimed to produce the recombinant P protein of norovirus and examine its blocking effect. The engineered strains expressed the P protein of NoVs GII.4, which showed binding capacity to histo-blood group antigens (HBGAs) through SDS-PAGE and ELISA. Rabbits were immunized to obtain neutralizing antibodies, which were found to block the binding of the P protein and human norovirus (HuNoV) to HBGAs. The recombinant P protein can induce antibodies to block HuNoV and murine norovirus (MNV) invasion.
Noroviruses (NoVs) are the main cause of acute gastroenteritis in all ages worldwide. The aim of this study was to produce the recombinant P protein of norovirus and to demonstrate its blocking effect. In this study, the engineered strains were induced to express the P protein of NoVs GII.4, which was identified using SDS-PAGE and ELISA as having the capacity to bind to histo-blood group antigens (HBGAs). Rabbits were immunized to obtain neutralizing antibodies. ELISA and ISC-RT-qPCR were used to determine the blocking efficacy of the neutralizing antibody to human norovirus (HuNoV) and murine norovirus (MNV). The recombinant P protein (35 KD) was obtained, and the neutralizing antibody was successfully prepared. The neutralizing antibody could block the binding of the P protein and HuNoV to HBGAs. Neutralizing antibodies can also block MNV invasion into host cells RAW264.7. The recombinant P protein expressed in E. coli can induce antibodies to block HuNoV and MNV. The recombinant P protein of NoVs GII.4 has the value of vaccine development.
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