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CRISPR-cas technology: A key approach for SARS-CoV-2 detection

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Publisher

FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2023.1158672

Keywords

CRISPR-Cas; nucleic acid detection; SARS-CoV-2; application; COVID-19

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The CRISPR system, originally discovered in prokaryotes, is an immune mechanism that clears foreign nucleic acids. Its strong ability of gene editing, regulation and detection in eukaryotes has made it extensively applied in basic and applied research. This article reviews the biology, mechanisms and relevance of CRISPR-Cas technology, as well as its applications in the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The CRISPR-Cas nucleic acid detection tools include CRISPR-Cas9, CRISPR-Cas12, CRISPR-Cas13, CRISPR-Cas14, CRISPR nucleic acid amplification detection technology, and CRISPR colorimetric readout detection system. The CRISPR technologies have been utilized in nucleic acid detection, including SARS-CoV-2 detection. Common nucleic acid detection based on CRISPR derivation technology include SHERLOCK, DETECTR, and STOPCovid. The CRISPR-Cas biosensing technology has been widely applied to point-of-care testing (POCT) by targeting recognition of both DNA molecules and RNA molecules.
The CRISPR (Clustered Regularly Spaced Short Palindromic Repeats) system was first discovered in prokaryotes as a unique immune mechanism to clear foreign nucleic acids. It has been rapidly and extensively used in basic and applied research owing to its strong ability of gene editing, regulation and detection in eukaryotes. Hererin in this article, we reviewed the biology, mechanisms and relevance of CRISPR-Cas technology and its applications in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) diagnosis. CRISPR-Cas nucleic acid detection tools include CRISPR-Cas9, CRISPR-Cas12, CRISPR-Cas13, CRISPR-Cas14, CRISPR nucleic acid amplification detection technology, and CRISPR colorimetric readout detection system. The above CRISPR technologies have been applied to the nucleic acid detection, including SARS-CoV-2 detection. Common nucleic acid detection based on CRISPR derivation technology include SHERLOCK, DETECTR, and STOPCovid. CRISPR-Cas biosensing technology has been widely applied to point-of-care testing (POCT) by targeting recognition of both DNA molecules and RNA Molecules.

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