Compressible viscoelasticity of cell membranes determined by gigahertz-frequency acoustic vibrations

Membrane viscosity is an important property of cell biology, which determines cellular function, development and disease progression. Various experimental and computational methods have been developed to investigate the mechanics of cells. However, there have been no experimental measurements of the membrane viscosity at high-frequencies in live cells. High frequency measurements are important because they can probe viscoelastic effects. Here, we investigate the membrane viscosity at gigahertz-frequencies through the damping of the acoustic vibrations of gold nanoplates. The experiments are modeled using a continuum mechanics theory which reveals that the membranes display viscoelasticity, with an estimated relaxation time of ca. 5.7+2.4/-2.7 ps. We further demonstrate that membrane viscoelasticity can be used to differentiate a cancerous cell line (the human glioblastoma cells LN-18) from a normal cell line (the mouse brain microvascular endothelial cells bEnd.3). The viscosity of cancerous cells LN-18 is lower than that of healthy cells bEnd.3 by a factor of three. The results indicate promising applications of characterizing membrane viscoelasticity at gigahertz-frequency in cell diagnosis.

Automated summary

Membrane viscosity is an important property of cell biology, and we investigated it at gigahertz frequencies through damping of acoustic vibrations of gold nanoplates. The experiments showed that cell membranes exhibit viscoelasticity with an estimated relaxation time. Furthermore, we demonstrated the potential of using membrane viscoelasticity at gigahertz frequencies to differentiate cancerous cells from normal cells, with cancer cells having lower viscosity compared to healthy cells. These findings have promising applications in cell diagnosis.