4.6 Article

A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs

Related references

Note: Only part of the references are listed.
Article Biophysics

A portable system for isothermal amplification and detection of exosomal microRNAs

Jingjing Qian et al.

Summary: This paper presents an on-chip exosomal miRNA amplification and detection system for rapid analysis, which quantitatively measures miRNAs extracted from exosomes using the EXPAR assay. The system is designed to allow storage of multiple oligonucleotide templates for the EXPAR, sample and reagent mixing, and simultaneous analysis of multiple exosomal miRNAs.

BIOSENSORS & BIOELECTRONICS (2022)

Article Multidisciplinary Sciences

MicroRNA sequence codes for small extracellular vesicle release and cellular retention

Ruben Garcia-Martin et al.

Summary: Research has shown that miRNAs have sorting sequences that determine their secretion in sEVs, different cell types preferentially use specific sorting sequences to define their sEV miRNA profile. Insertion or deletion of these sequences affects miRNA secretion. RNA-binding proteins Alyref and Fus are involved in the mechanism, leading to enhanced miRNA delivery and inhibition of target genes in distant cells.

NATURE (2022)

Article Multidisciplinary Sciences

Selection of reference genes for quantitative analysis of microRNA expression in three different types of cancer

Yuliya A. Veryaskina et al.

Summary: miRNAs are important biomarkers in cancer research, and qPCR is the most commonly used technique for measuring miRNA expression levels, but normalization against reference genes is required. This study identified miRNAs with stable expression in different tissues, which may serve as promising reference genes for miRNA quantification in qPCR in the future.

PLOS ONE (2022)

Article Chemistry, Multidisciplinary

A Highly Sensitive CRISPR-Empowered Surface Plasmon Resonance Sensor for Diagnosis of Inherited Diseases with Femtomolar-Level Real-Time Quantification

Fei Zheng et al.

Summary: The novel CRISPR-empowered SPR sensing platform developed in this study combines CRISPR technology with SPR sensor technology to enable rapid, precise, sensitive, and specific detection of target gene sequences.

ADVANCED SCIENCE (2022)

Article Engineering, Biomedical

Fast and sensitive detection of SARS-CoV-2 RNA using suboptimal protospacer adjacent motifs for Cas12a

Shuhan Lu et al.

Summary: This study presents a one-step fluorescence assay for the detection of SARS-CoV-2 RNA in nasopharyngeal samples. The assay offers a short detection time and sensitivity comparable to RT-qPCR, while also allowing for flexible assay design.

NATURE BIOMEDICAL ENGINEERING (2022)

Article Engineering, Biomedical

A paper-based assay for the colorimetric detection of SARS-CoV-2 variants at single-nucleotide resolution

Ting Zhang et al.

Summary: This study presents a paper-based assay for the rapid colorimetric detection of SARS-CoV-2 variants. The assay utilizes nucleic acid reactions and enzymatic amplification to detect different variants of the virus within 30 minutes. The results show 100% concordance with other commonly used detection methods. This customizable and inexpensive paper-based assay could facilitate viral surveillance.

NATURE BIOMEDICAL ENGINEERING (2022)

Article Engineering, Biomedical

Rapid detection of SARS-CoV-2 RNA in saliva via Cas13

Sita S. Chandrasekaran et al.

Summary: This article reports a COVID-19 testing method implemented in a microfluidic device, which can rapidly detect SARS-CoV-2 RNA in saliva samples using isothermal RNA amplification and Cas13-mediated cleavage of a quenched fluorophore. The method has high sensitivity and specificity, and the testing can be completed within 60 minutes.

NATURE BIOMEDICAL ENGINEERING (2022)

Article Biophysics

Recent advances in the rapid detection of microRNA with lateral flow assays

Nan Wang et al.

Summary: MicroRNAs (miRNA) are endogenous non-coding small molecule RNAs that play a crucial role in gene regulation and biological functions. Lateral flow assay is a rapid and accurate detection method that has been widely used in medical diagnosis, agriculture, food, and environmental safety.

BIOSENSORS & BIOELECTRONICS (2022)

Review Chemistry, Multidisciplinary

Nucleic Acid Aptamers for Molecular Diagnostics and Therapeutics: Advances and Perspectives

Long Li et al.

Summary: SELEX technology has shown the ability to evolve artificial ligands with affinity and specificity, and aptamers complement antibodies with unique advantages such as small size, low cost, and facile chemical modification, making them a potential molecular tool for future clinical needs in biomedicine.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

Review Chemistry, Analytical

Advances in Analytical Technologies for Extracellular Vesicles

He Yan et al.

ANALYTICAL CHEMISTRY (2021)

Article Multidisciplinary Sciences

Isolation of extra-cellular vesicles in the context of pancreatic adenocarcinomas: Addition of one stringent filtration step improves recovery of specific microRNAs

Yi-Fan Xu et al.

Summary: A simple protocol was developed to separate PDAC EVs into three groups, among which the OP1 group showed elevated levels of miR-196a and miR-1246, indicating their potential as biomarkers for PDAC.

PLOS ONE (2021)

Article Chemistry, Analytical

A one-step, one-pot CRISPR nucleic acid detection platform (CRISPR-top): Application for the diagnosis of COVID-19

Shijun Li et al.

Summary: CRISPR-top is a novel diagnostic method that integrates target pre-amplification and CRISPR-mediated detection in one reaction mixture for SARS-CoV-2 detection. This simple, rapid, accurate, and highly sensitive method can return results within 60 minutes, making it suitable for clinical and resource-challenged settings.

TALANTA (2021)

Article Multidisciplinary Sciences

Systematic evaluation of multiple qPCR platforms, NanoString and miRNA-Seq for microRNA biomarker discovery in human biofluids

Lewis Z. Hong et al.

Summary: Aberrant miRNA expression is associated with various diseases, and extracellular miRNAs circulating in the bloodstream are stable and potential non-invasive biomarkers for early disease detection. The challenge lies in the low abundance of miRNA in biofluids, but using miRNA-Seq for discovery and targeted qPCR for validation seems to be a rational strategy for miRNA biomarker development in clinical samples with limited biofluids.

SCIENTIFIC REPORTS (2021)

Review Oncology

The Role of Exosomes in Pancreatic Cancer From Bench to Clinical Application: An Updated Review

Kai Chen et al.

Summary: Pancreatic ductal adenocarcinoma (PDAC) is a gastrointestinal malignancy with a low survival rate, and exosomes have potential roles in PDAC treatment strategies. Exosomes can serve as biomarkers and drug carriers in PDAC clinical applications, but obstacles still need to be overcome.

FRONTIERS IN ONCOLOGY (2021)

Article Chemistry, Analytical

CRISPR/Cas12a-Assisted Ligation-Initiated Loop-Mediated Isothermal Amplification (CAL-LAMP) for Highly Specific Detection of microRNAs

Mai Zhang et al.

Summary: A CRISPR/Cas12a-assisted sequence-specific detection method for LAMP products was developed to eliminate non-specific amplification effects. By designing target-specific stem-loop DNA probes, primer design for LAMP was greatly simplified, achieving sensitive detection of miRNAs with high specificity.

ANALYTICAL CHEMISTRY (2021)

Review Medicine, General & Internal

Pancreatic Cancer: A Review

Wungki Park et al.

Summary: Pancreatic ductal adenocarcinoma (PDAC) is a relatively uncommon cancer with increasing incidence each year, resulting in a high percentage of patients being diagnosed with advanced disease. Current cytotoxic therapies for PDAC are moderately effective, emphasizing the importance of multidisciplinary management, comprehensive germline testing, and integrated supportive care for all patients.

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION (2021)

Article Multidisciplinary Sciences

Single-molecule amplification-free multiplexed detection of circulating microRNA cancer biomarkers from serum

Shenglin Cai et al.

Summary: The study presents a new sensing platform for miRNA detection, offering high sensitivity and specificity directly in human serum, and demonstrating monitoring of miR-141-3p and miR-375-3p in various stages of prostate cancer.

NATURE COMMUNICATIONS (2021)

Review Oncology

Non-Invasive Biomarkers for Earlier Detection of Pancreatic Cancer-A Comprehensive Review

Greta Brezgyte et al.

Summary: Pancreatic ductal adenocarcinoma (PDAC) is an aggressive and lethal disease, with challenges in accurately detecting it at an early stage using current biomarkers. This study systematically reviewed the literature on non-invasive biomarkers for early PDAC detection over a 10-year period, identifying promising biomarkers but highlighting the complexity in translating them to clinical use.

CANCERS (2021)

Review Engineering, Biomedical

CRISPR-based diagnostics

Michael M. Kaminski et al.

Summary: This Review summarizes the rapidly expanding toolbox of CRISPR-based diagnostics, highlighting their applications in real-time diagnosis. It mentions that nucleic acid-based diagnostics are sensitive and specific, but most require expensive equipment and trained personnel.

NATURE BIOMEDICAL ENGINEERING (2021)

Article Cell Biology

Extracellular Vesicle-Based Detection of Pancreatic Cancer

Yesim Verel-Yilmaz et al.

Summary: The study aimed to determine whether ADAM8-positive extracellular vesicles (EVs) and cargo microRNAs could distinguish PDAC or its precursor lesions from healthy controls. FACS analysis and EV cargo analysis of miRNAs revealed a potential diagnostic biomarker set to discriminate PDAC and precursor lesions from healthy controls.

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY (2021)

Review Oncology

Early screening and diagnosis strategies of pancreatic cancer: a comprehensive review

Jinshou Yang et al.

Summary: Pancreatic cancer is a highly malignant tumor with poor prognosis, often diagnosed at advanced stages due to lack of early symptoms. Early diagnosis through screening and understanding risk factors is crucial. Current biomarkers like CA19-9 have limited specificity, driving research towards discovering novel biomarkers and utilizing innovative technologies for improved detection.

CANCER COMMUNICATIONS (2021)

Article Biochemistry & Molecular Biology

Serum miRNA Profiling for Early PDAC Diagnosis and Prognosis: A Retrospective Study

Ada Aita et al.

Summary: Analysis of serum miRNAs in pancreatic cancer patients identified potential biomarkers for early diagnosis and prognosis prediction. Some miRNAs were associated with lymph-node metastases and cancer progression pathways.

BIOMEDICINES (2021)

Review Chemistry, Multidisciplinary

Advances in multiplexed techniques for the detection and quantification of microRNAs

Thomas Jet et al.

Summary: MicroRNA detection is a crucial challenge in analytical chemistry, with the need for high sensitivity, specificity, robustness, and quantitative analysis. The ability to perform multiplexed detection of miRNA concentrations is important for recognizing disease signatures and new innovative detection methods have been developed to address this challenge.

CHEMICAL SOCIETY REVIEWS (2021)

Review Multidisciplinary Sciences

The biology, function, and biomedical applications of exosomes

Raghu Kalluri et al.

SCIENCE (2020)

Article Gastroenterology & Hepatology

Exosomal miRNA signatures of pancreatic lesions

Caterina Vicentini et al.

BMC GASTROENTEROLOGY (2020)

Review Cell Biology

RNA delivery by extracellular vesicles in mammalian cells and its applications

Killian O'Brien et al.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2020)

Article Biochemical Research Methods

Accurate MRSA identification through dual-functional aptamer and CRISPR-Cas12a assisted rolling circle amplification

Liqi Xu et al.

JOURNAL OF MICROBIOLOGICAL METHODS (2020)

Letter Medicine, General & Internal

Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing

Julia Joung et al.

NEW ENGLAND JOURNAL OF MEDICINE (2020)

Article Multidisciplinary Sciences

Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay

Xiong Ding et al.

NATURE COMMUNICATIONS (2020)

Review Biochemistry & Molecular Biology

Extracellular Vesicles as Potential Biomarkers for Early Detection and Diagnosis of Pancreatic Cancer

Nelson S. Yee et al.

BIOMEDICINES (2020)

Article Chemistry, Analytical

CRISPR-Cas12a enhanced rolling circle amplification method for ultrasensitive miRNA detection

Gong Zhang et al.

MICROCHEMICAL JOURNAL (2020)

Review Oncology

The evolving translational potential of small extracellular vesicles in cancer

Andreas Moller et al.

NATURE REVIEWS CANCER (2020)

Article Chemistry, Analytical

A Cas12a-mediated cascade amplification method for microRNA detection

Huan-Huan Sun et al.

ANALYST (2020)

Article Chemistry, Analytical

MicroRNA Detection Specificity: Recent Advances and Future Perspective

Tinglan Ouyang et al.

ANALYTICAL CHEMISTRY (2019)

Article Engineering, Biomedical

Ultrasensitive detection of circulating exosomes with a 3D-nanopatterned microfluidic chip

Peng Zhang et al.

NATURE BIOMEDICAL ENGINEERING (2019)

Article Engineering, Biomedical

Detection of unamplified target genes via CRISPR-Cas9 immobilized on a graphene field-effect transistor

Reza Hajian et al.

NATURE BIOMEDICAL ENGINEERING (2019)

Article Biochemical Research Methods

HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation

Linxian Li et al.

ACS SYNTHETIC BIOLOGY (2019)

Review Biochemical Research Methods

qPCR-based methods for expression analysis of miRNAs

Diego A. Forero et al.

BIOTECHNIQUES (2019)

Review Cell Biology

Extracellular miRNAs: From Biomarkers to Mediators of Physiology and Disease

Marcelo A. Mori et al.

CELL METABOLISM (2019)

Article Cell Biology

Assessment of methods for serum extracellular vesicle small RNA sequencing to support biomarker development

Swetha Srinivasan et al.

JOURNAL OF EXTRACELLULAR VESICLES (2019)

Review Medicine, Research & Experimental

MicroRNA amplification and detection technologies: opportunities and challenges for point of care diagnostics

Vivek Priy Dave et al.

LABORATORY INVESTIGATION (2019)

Review Biotechnology & Applied Microbiology

CRISPR/Cas Systems towards Next-Generation Biosensing

Yi Li et al.

TRENDS IN BIOTECHNOLOGY (2019)

Review Cell Biology

Exosomal miRNAs as novel cancer biomarkers: Challenges and opportunities

Mahsa Salehi et al.

JOURNAL OF CELLULAR PHYSIOLOGY (2018)

Review Oncology

Clinical utility of circulating non-coding RNAs - an update

Simone Anfossi et al.

NATURE REVIEWS CLINICAL ONCOLOGY (2018)

Review Cell Biology

Shedding light on the cell biology of extracellular vesicles

Guillaume van Niel et al.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2018)

Article Multidisciplinary Sciences

Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a)

Digvijay Singh et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2018)

Article Multidisciplinary Sciences

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

Janice S. Chen et al.

SCIENCE (2018)

Article Multidisciplinary Sciences

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

Jonathan S. Gootenberg et al.

SCIENCE (2018)

Review Biotechnology & Applied Microbiology

Biosensors Using Hybridization Chain Reaction - Design and Signal Amplification Strategies of Hybridization Chain Reaction

Chae Rin Park et al.

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING (2018)

Article Multidisciplinary Sciences

Nucleic acid detection with CRISPR-Cas13a/C2c2

Jonathan S. Gootenberg et al.

SCIENCE (2017)

Article Multidisciplinary Sciences

Global temporal patterns of pancreatic cancer and association with socioeconomic development

Martin C. S. Wong et al.

SCIENTIFIC REPORTS (2017)

Article Cell Biology

A comprehensive method for identification of suitable reference genes in extracellular vesicles

Kenneth Gouin et al.

JOURNAL OF EXTRACELLULAR VESICLES (2017)

Review Chemistry, Multidisciplinary

Isothermal Amplification for MicroRNA Detection: From the Test Tube to the Cell

Ruijie Deng et al.

ACCOUNTS OF CHEMICAL RESEARCH (2017)

Article Biophysics

A microarray platform for detecting disease-specific circulating miRNA in human serum

Somenath Roy et al.

BIOSENSORS & BIOELECTRONICS (2016)

Article Biochemistry & Molecular Biology

Sensitive and specific miRNA detection method using SplintR Ligase

Jingmin Jin et al.

NUCLEIC ACIDS RESEARCH (2016)

Article Chemistry, Multidisciplinary

Simple Isothermal Strategy for Multiplexed, Rapid, Sensitive, and Accurate miRNA Detection

Eugene J. H. Wee et al.

ACS SENSORS (2016)

Article Cell Biology

Comparative analysis of EV isolation procedures for miRNAs detection in serum samples

Zoraida Andreu et al.

JOURNAL OF EXTRACELLULAR VESICLES (2016)

Article Chemistry, Analytical

MicroRNA Detection: Current Technology and Research Strategies

Eric A. Hunt et al.

ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 8 (2015)

Article Chemistry, Medicinal

The Role of miR-21 in Cancer

Susan R. Pfeffer et al.

DRUG DEVELOPMENT RESEARCH (2015)

Article Chemistry, Multidisciplinary

Live Cell MicroRNA Imaging Using Cascade Hybridization Reaction

Zoya Cheglakov et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2015)

Article Medicine, Research & Experimental

Primary outgrowth cultures are a reliable source of human pancreatic stellate cells

Song Han et al.

LABORATORY INVESTIGATION (2015)

Review Oncology

MicroRNA biogenesis pathways in cancer

Shuibin Lin et al.

NATURE REVIEWS CANCER (2015)

Article Chemistry, Multidisciplinary

Toehold-initiated Rolling Circle Amplification for Visualizing Individual MicroRNAs In Situ in Single Cells

Ruijie Deng et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2014)

Review Pathology

MicroRNAs in Cancer

Gianpiero Di Leva et al.

ANNUAL REVIEW OF PATHOLOGY: MECHANISMS OF DISEASE, VOL 9 (2014)

Article Biochemistry & Molecular Biology

U6 is not a suitable endogenous control for the quantification of circulating microRNAs

Mengqin Xiang et al.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2014)

Review Chemistry, Multidisciplinary

Rolling circle amplification: a versatile tool for chemical biology, materials science and medicine

M. Monsur Ali et al.

CHEMICAL SOCIETY REVIEWS (2014)

Article Computer Science, Artificial Intelligence

Leave-one-out cross-validation is risk consistent for lasso

Darren Homrighausen et al.

MACHINE LEARNING (2014)

Review Cell Biology

Regulation of microRNA biogenesis

Minju Ha et al.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2014)

Article Biochemistry & Molecular Biology

Efficient DNA ligation in DNA-RNA hybrid helices by Chlorella virus DNA ligase

Gregory J. S. Lohman et al.

NUCLEIC ACIDS RESEARCH (2014)

Article Multidisciplinary Sciences

Quantitative and stoichiometric analysis of the microRNA content of exosomes

John R. Chevillet et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2014)

Article Biochemistry & Molecular Biology

Evaluation of Three RT-qPCR-Based miRNA Detection Methods Using Seven Rice miRNAs

Guiping Mou et al.

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY (2013)

Review Immunology

miRNA profiling for biomarker discovery in multiple sclerosis: From microarray to deep sequencing

Mireia Guerau-de-Arellano et al.

JOURNAL OF NEUROIMMUNOLOGY (2012)

Article Chemistry, Multidisciplinary

Ultrasensitive Detection of microRNAs by Exponential Isothermal Amplification

Hongxia Jia et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2010)

Article Medical Laboratory Technology

The MicroRNA Spectrum in 12 Body Fluids

Jessica A. Weber et al.

CLINICAL CHEMISTRY (2010)

Article Biotechnology & Applied Microbiology

Programmable in situ amplification for multiplexed imaging of mRNA expression

Harry M. T. Choi et al.

NATURE BIOTECHNOLOGY (2010)

Review Cell Biology

MicroRNA control of signal transduction

Masafumi Inui et al.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2010)

Article Medicine, General & Internal

MicroRNA expression patterns to differentiate pancreatic adenocarcinoma from normal pancreas and chronic pancreatitis

Mark Bloomston et al.

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION (2007)

Article Biochemical Research Methods

Detecting antigens by quantitative immuno-PCR

Christof M. Niemeyer et al.

NATURE PROTOCOLS (2007)

Article Multidisciplinary Sciences

Circle-to-circle amplification for precise and sensitive DNA analysis

F Dahl et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2004)

Review Immunology

Exosomes:: Composition, biogenesis and function

C Théry et al.

NATURE REVIEWS IMMUNOLOGY (2002)

Article Biochemistry & Molecular Biology

Signal amplification by rolling circle amplification on DNA microarrays

G Nallur et al.

NUCLEIC ACIDS RESEARCH (2001)