Screening of sensitivity to mandipropamid of Plasmopara viticola populations from Italian vineyards by molecular and biological methods

The objective of this work was to study the sensitivity to mandipropamid of 33 Plasmopara viticola populations utilizing both molecular and biological techniques. The PCR-RFLP technique was developed in order to detect the single point mutation, G1105S, occurring on the PvCesA3 gene. The sensitivity was also studied using the leaf-disc bioassay. Thirty-three downy mildew-infected leaf samples, collected from 2010 to 2013 from Italian vineyards, were used in the study. PCR-RFLP revealed the presence of 7 resistant, 12 sensitive, 14 mixed (sensitive and resistant) mutation profiles. Effective concentration for 50% inhibition rate (EC50) calculated from the bioassays showed an EC50 <1 mg l(-1) for samples that showed sensitive profiles, while for those samples that had a mixed profile, EC50 ranged from <1 to >300 mg l(-1), and values for resistant profiles ranged from 200.28 to >300 mg l(-1). The results suggest that P. viticola populations infecting Italian vineyards are under a selection pressure due to CAA-based fungicide applications.