Antioxidant, anti-inflammatory, and anticancer function of Engleromyces goetzei Henn aqueous extract on human intestinal Caco-2 cells treated with t-BHP

High body mass index (high BMI, obesity) is a serious public health problem, and obesity-induced oxidative stress, inflammation, and cancer have become modern epidemic diseases. We carried out this study to explore a functional beverage that may protect against obesity-induced diseases. The Engleromyces goetzei Henn herbal tea is such a candidate. For this study, we carried out LC-MS analysis of E. goetzei Henn aqueous extract (EgH-AE); then used the Caco-2 cell line for the model cells and treated the cells with t-BHP to form an oxidative stress system. An MTT assay was used for testing the biocompatibility and cytoprotective effects; reactive oxygen species and malondialdehyde determination was used for evaluating the antioxidative stress effect; TNF-a and IL-1b were used for observing the anti-inflammatory effect, and 8-OHdG for monitoring anticancer activity. The results of this study demonstrate that the EgH-AE has very good biocompatibility with the Caco-2 cell line and has good cytoprotective, antioxidant, anti-inflammatory, and anticancer properties. It is clear that EgH-AE, a kind of ancient herbal tea, may be used to develop a functional beverage that can be given to people with a high BMI to protect against obesity-induced diseases.

Automated summary

High body mass index (BMI) is a major public health problem, leading to obesity-induced oxidative stress, inflammation, and cancer. In this study, the researchers investigated the potential of Engleromyces goetzei Henn herbal tea as a functional beverage to protect against these diseases. The study involved analyzing the herbal tea extract, testing its biocompatibility and cytoprotective effects on Caco-2 cells, evaluating its antioxidative stress, anti-inflammatory, and anticancer properties. The results demonstrate that the herbal tea extract shows promise in preventing obesity-induced diseases in individuals with high BMI.