Dynamic Deformation Measurement of an Intact Single Cell via Microfluidic Chip with Integrated Liquid Exchange

This paper reports a method to measure the mechanical properties of a single cell using a microfluidic chip with integrated force sensing and a liquid exchange function. A single cell is manipulated and positioned between a pushing probe and a force sensor probe using optical tweezers. These two on-chip probes were designed to capture and deform the cells. The single cell is deformed by moving the pushing probe, which is driven by an external force. The liquid-liquid interface is formed between the probes by laminar flow to change the extracellular environment. The position of the interface is shifted by controlling the injection pressure. Two positive pressures and one negative pressure are adjusted to balance the diffusion and perturbation of the flow. The mechanical properties of a single Synechocystis sp. strain PCC 6803 were measured in different osmotic concentration environments in the microfluidic chip. The liquid exchange was achieved in approximately 0.3-0.7 s, and the dynamic deformation of a single cell was revealed simultaneously. Measurements of two Young's modulus values under alterable osmotic concentrations and the dynamic response of a single cell in osmotic shock can be collected within 30 s. Dynamic deformations of wild-type (WT) and mutant Synechocystis cells were investigated to reveal the functional mechanism of mechanosensitive (MS) channels. This system provides a novel method for monitoring the real-time mechanical dynamics of a single intact cell in response to rapid external osmotic changes; thus, it opens up novel opportunities for characterizing the accurate physiological function of MS channels in cells. (c) 2023 THE AUTHORS. Published by Elsevier LTD on behalf of Chinese Academy of Engineering and Higher Education Press Limited Company. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Automated summary

This paper presents a method for measuring the mechanical properties of a single cell using a microfluidic chip. The cell is manipulated and deformed using optical tweezers, and the extracellular environment is changed through liquid exchange. The method allows for rapid measurements of the mechanical properties and dynamic response of a single cell in different osmotic concentration environments.