Molecular and cellular evolution of the amygdala across species analyzed by single-nucleus transcriptome profiling

The amygdala, or an amygdala-like structure, is found in the brains of all vertebrates and plays a critical role in survival and reproduction. However, the cellular architecture of the amygdala and how it has evolved remain elusive. Here, we generated single-nucleus RNA-sequencing data for more than 200,000 cells in the amygdala of humans, macaques, mice, and chickens. Abundant neuronal cell types from different amygdala subnuclei were identified in all datasets. Cross-species analysis revealed that inhibitory neurons and inhibitory neuron-enriched subnuclei of the amygdala were well-conserved in cellular composition and marker gene expression, whereas excitatory neuron-enriched subnuclei were relatively divergent. Furthermore, LAMP5(+) interneurons were much more abundant in primates, while DRD2(+) inhibitory neurons and LAMP5(+)SATB2(+) excitatory neurons were dominant in the human central amygdalar nucleus (CEA) and basolateral amygdalar complex (BLA), respectively. We also identified CEA-like neurons and their species-specific distribution patterns in chickens. This study highlights the extreme cell-type diversity in the amygdala and reveals the conservation and divergence of cell types and gene expression patterns across species that may contribute to species-specific adaptations.

Automated summary

By analyzing RNA sequencing data from over 200,000 neurons in humans, macaques, mice, and chickens, this study reveals the precise conservation and relative divergence of cellular architecture in the amygdala. It highlights the importance of cell types and gene expression in amygdala for species evolution and specific adaptations.