Journal
CELLS
Volume 12, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/cells12121577
Keywords
amniotic fluid cells; single-cell RNA sequencing; spina bifida; amniotic fluid stem cells
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This study characterized the tissue origin and marker expression of cultured amniotic fluid cells at the single-cell level using single-cell RNA sequencing. It revealed the heterogeneity of amniotic fluid cells and identified cell types of neural origin in fetuses with spina bifida aperta. The findings provide valuable information for further research and clinical applications of amniotic fluid cells.
Amniotic fluid has been proposed as an easily available source of cells for numerous applications in regenerative medicine and tissue engineering. The use of amniotic fluid cells in biomedical applications necessitates their unequivocal characterization; however, the exact cellular composition of amniotic fluid and the precise tissue origins of these cells remain largely unclear. Using cells cultured from the human amniotic fluid of fetuses with spina bifida aperta and of a healthy fetus, we performed single-cell RNA sequencing to characterize the tissue origin and marker expression of cultured amniotic fluid cells at the single-cell level. Our analysis revealed nine different cell types of stromal, epithelial and immune cell phenotypes, and from various fetal tissue origins, demonstrating the heterogeneity of the cultured amniotic fluid cell population at a single-cell resolution. It also identified cell types of neural origin in amniotic fluid from fetuses with spina bifida aperta. Our data provide a comprehensive list of markers for the characterization of the various progenitor and terminally differentiated cell types in cultured amniotic fluid. This study highlights the relevance of single-cell analysis approaches for the characterization of amniotic fluid cells in order to harness their full potential in biomedical research and clinical applications.
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