Journal
CELLS
Volume 12, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/cells12060912
Keywords
mitochondria; ATP synthase; haemoglobin; hypoxia; ATP synthase inhibitory factor 1
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This study investigated the interaction of mitochondrial haemoglobin with ATP synthase and its associated proteins, and found upregulated expression of mitochondrial haemoglobin in response to hypoxia. The results suggest that mitochondrial pH and ATP synthase activity are involved in the mitochondrial haemoglobin response to hypoxia.
The globin protein superfamily has diverse functions. Haemoglobin has been found in non-erythroid locations, including within the mitochondria. Using co-immunoprecipitation and in silico methods, we investigated the interaction of mitochondrial haemoglobin with ATP synthase and its associated proteins, including inhibitory factor 1 (IF1). We measured the expression of mitochondrial haemoglobin in response to hypoxia. In vitro and in silico evidence of interactions between mitochondrial haemoglobin and ATP synthase were found, and we report upregulated mitochondrial haemoglobin expression in response to hypoxia within skeletal muscle tissue. Our observations indicate that mitochondrial pH and ATP synthase activity are implicated in the mitochondrial haemoglobin response to hypoxia.
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