4.6 Article

The aMSH-Dependent PI3K Pathway Supports Energy Metabolism, via Glucose Uptake, in Melanoma Cells

Journal

CELLS
Volume 12, Issue 7, Pages -

Publisher

MDPI
DOI: 10.3390/cells12071099

Keywords

melanoma; PI3K pathway; alpha MSH; glucose uptake; energy metabolism

Categories

Ask authors/readers for more resources

Stimulation of melanocytes and murine melanoma cells with aMSH plus the PI3K inhibitor LY294002 resulted in ROS increase, oxidative DNA damage, and pigment retention. Treatment with aMSH plus LY294002 altered cortical actin architecture and reduced ATP levels. The aMSH-activated PI3K pathway modulated energy metabolism by influencing glucose uptake and mitochondrial mass, leading to changes in cell motility and potential therapeutic strategies.
Stimulation of melanocytes and murine melanoma cells with aMSH plus the PI3K inhibitor LY294002 resulted in ROS increase, oxidative DNA damage, and pigment retention. We performed cellular and molecular biology assays (Western blot, FACS, immunofluorescence analysis, scratch assay) on murine and human melanoma cells. Treatment with aMSH plus LY294002 altered cortical actin architecture. Given that cytoskeleton integrity requires energy, we next evaluated ATP levels and we observed a drop in ATP after exposure to aMSH plus LY294002. To evaluate if the aMSH-activated PI3K pathway could modulate energy metabolism, we focused on glucose uptake by analyzing the expression of the Glut-1 glucose translocator. Compared with cells treated with aMSH alone, those exposed to combined treatment showed a reduction of Glut-1 on the plasma membrane. This metabolic alteration was associated with changes in mitochondrial mass. A significant decrease of the cell migratory potential was also observed. We demonstrated that the aMSH-dependent PI3K pathway acts as a regulator of energy metabolism via glucose uptake, influencing the actin cytoskeleton, which is involved in melanosome release and cell motility. Hence, these results could constitute the basis for innovative therapeutical strategies.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available