Journal
CELLS
Volume 12, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/cells12060907
Keywords
Chlamydomonas; cell wall; 2,6-dichloro-1; 4-benzoquinone (DCBQ); photosystem II; O-2 evolution rate
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Chlamydomonas reinhardtii is commonly used in studies on green algae to investigate photosynthesis and biotechnological approaches. The study found that using low concentrations of 2,6-dichloro-1,4-benzoquinone (DCBQ) paired with potassium ferricyanide (FeCy) is necessary to achieve the maximum rate of O-2 evolution. Higher concentrations of DCBQ can strongly suppress O-2 evolution. The concentration of DCBQ required depends on the presence of a cell wall, with 0.1 mM for cells with a cell wall and 0.2-0.4 mM for cells without a cell wall.
Chlamydomonas reinhardtii is a widely used object in studies on green algae concerning both photosynthesis aspects and possible biotechnological approaches. The measurement of the maximum O-2 evolution by photosystem II (PSII) in living algal cells in the presence of artificial acceptors is one of the commonly used methods for determining the photosynthetic apparatus state or its change as compared to a control, parent strain, etc., because PSII is the most sensitive component of the thylakoid membrane. The present study shows the need to use low concentrations of 2,6-dichloro-1,4-benzoquinone (DCBQ) paired with potassium ferricyanide (FeCy) for achieving the maximum O-2 evolution rate, while a DCBQ concentration above certain threshold results in strong suppression of O-2 evolution. The required DCBQ concentration depends on the presence of the cell wall and should be exactly similar to 0.1 mM or in the range of 0.2-0.4 mM for cells with and without a cell wall, respectively. The inhibition effect is caused, probably, by a higher content of DCBQ in the oxidized form inside cells; this depends on the presence of the cell wall, which influences the efficiency of DCBQ diffusion into and out of the cell, where it is maintained by FeCy in the oxidized state. The possible mechanism of DCBQ inhibition action is discussed.
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