Tumor-Infiltrating CD45RO+ Memory Cells Are Associated with Favorable Prognosis in Oral Squamous Cell Carcinoma Patients

Simple Summary Tumor-infiltrating lymphocytes (TILs) have long been used to predict the prognosis of solid tumors. TIL levels have been reported to be closely associated with the prognosis of patients with squamous cell carcinoma. In contrast, major histocompatibility complex (MHC) class I chain-related molecule A (MICA), which acts as a ligand for NKG2D in natural killer (NK) cells and CD8+ T cells, is related to a higher survival rate in patients with oral squamous cell carcinoma (OSCC). In this study, we investigated the association of OSCC with TILs and MICA in patients with OSCC and its potential as a biomarker. Background: Tumor-infiltrating lymphocytes (TILs) have been used to predict the prognosis of solid tumors. In this study, we investigated which molecules in TILs play a role in the prognosis of patients with oral squamous cell carcinoma (OSCC). Methods: In a retrospective case-control study, we immunohistochemically evaluated the expression of CD3, CD8, CD45RO, Granzyme B, and the major histocompatibility complex class I chain-related molecule A (MICA) of the histocompatibility complex as predictors of prognosis in 33 patients with OSCC. The patients were classified as TILs(High) or TILs(Low) according to the number of TILs for each molecule in the central tumor (CT) and invasive margin (IM). Furthermore, MICA expression scores were determined based on the intensity of the staining. Results: CD45RO(+)/TIL in the nonrecurrent group were significantly higher than those in the recurrent group in the CT and IM areas (p < 0.05). The disease-free survival/overall survival rate of the CD45RO(+)/TILs(Low) group in the CT and IM areas and the Granzyme B+/TILs(Low) group in the IM area was significantly lower than that of the CD45RO(+)/TILs(High) group and the Granzyme B+/TILs(High) group, respectively (p < 0.05). Furthermore, the MICA expression score of tumors around the CD45RO(+)/TILs(High) group was significantly higher than that of the CD45RO(+)/TILs(Low) group (p < 0.05). Conclusions: A high ratio of CD45RO-expressing TILs was associated with a disease-free/overall survival improvement in OSCC patients. Furthermore, the number of TILs that express CD45RO was associated with the expression of MICA in tumors. These results suggest that CD45RO-expressing TILs are useful biomarkers for OSCC.

Automated summary

This study investigated the association of tumor-infiltrating lymphocytes (TILs) and MICA with oral squamous cell carcinoma (OSCC) and their potential as biomarkers. The results showed that a high ratio of CD45RO-expressing TILs was associated with improved disease-free/overall survival in OSCC patients, while a low ratio of CD45RO-expressing TILs was associated with poorer survival. Furthermore, the expression of MICA in tumors was related to the proportion of CD45RO-expressing TILs. Therefore, CD45RO-expressing TILs can serve as biomarkers for predicting the prognosis of OSCC.