A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics

We introduce a cost-effective, robust high-throughput-compatible plasma depletion method enabling in-depth profiling of plasma that detects >1300 proteins per run with a throughput of 60 samples per day. The method has been fully validated by processing >3000 samples with no apparent batch effect at a cost for the depletion step of similar to$2.5 per sample.

Automated summary

We present a cost-effective, robust high-throughput-compatible method for depleting plasma, enabling comprehensive profiling of plasma with detection of >1300 proteins per run and throughput of 60 samples per day. The method has been extensively validated, processing >3000 samples with no apparent batch effect, and costs around $2.5 per sample for the depletion step.