Journal
SCIENCE ADVANCES
Volume 9, Issue 14, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.adh0411
Keywords
-
Categories
Ask authors/readers for more resources
During metazoan development, there is a marked change in developmental potential from the parental germline to the embryo, and this study aims to understand how the next life cycle is reset. Through gene editing techniques, the study investigates the expression pattern and role of individual RC histone H3 genes in Caenorhab-ditis elegans and compares them to the histone variant, H3.3. The findings reveal a tightly regulated epigenome landscape change from the germline to embryos, showing that a change from a H3.3-to H3-enriched epigenome restricts developmental plasticity and uncovers distinct roles for individual H3 genes in regulating germline chromatin.
During metazoan development, the marked change in developmental potential from the parental germline to the embryo raises an important question regarding how the next life cycle is reset. As the basic unit of chroma-tin, histones are essential for regulating chromatin structure and function and, accordingly, transcription. However, the genome-wide dynamics of the canonical, replication-coupled (RC) histones during gametogenesis and embryogenesis remain unknown. In this study, we use CRISPR-Cas9-mediated gene editing in Caenorhab-ditis elegans to investigate the expression pattern and role of individual RC histone H3 genes and compare them to the histone variant, H3.3. We report a tightly regulated epigenome landscape change from the germline to embryos that are regulated through differential expression of distinct histone gene clusters. Together, this study reveals that a change from a H3.3-to H3-enriched epigenome during embryogenesis restricts developmental plasticity and uncovers distinct roles for individual H3 genes in regulating germline chromatin.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available