Journal
LANGMUIR
Volume 32, Issue 45, Pages 11763-11770Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.langmuir.6b03016
Keywords
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Funding
- National Key Technology R&D Program of China [2012BAI16B02]
- National Natural Science Foundation of China [81171416, 60875078, 21174127, 21474085]
- Zhejiang Provincial Natural Science Foundation of China [LZ13E030001]
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In this study, a versatile fabrication method for coating enzyme-based biosensors With ultrathin antifouling zwitterionic polymer films to meet the challenge of the long-time stability of sensors in vivo was developed. Electrochemically mediated atom transfer radical polymerization (eATRP) was applied to polymerize zwitterionic sulfobetaine methacrylate monomers on the rough enzyme-absorbed electrode surfaces; meanwhile, a refined overall bromination was developed to improve the coverage of polymers on the biosensor surfaces and to maintain the enzyme activity simultaneously for the first time. X-ray photoelectron spectroscopy and atomic force microscopy were used to characterize the properties of the polymer layers. The antifouling performance and long-time stability in 37 degrees C undiluted bovine serum in vitro were evaluated. The results showed that the polymer brush coatings diminished over 99% nonspecific protein adsorption, and that the sensitivity of the evaluated sensor was maintained at 94% after 15 days. The overall sensitivity deviation of 7% was nearly 50% lower than that of the polyurethane-coated ones and also much smaller than the current commercially available glucose biosensors. The results suggested that this highly controllable electrodeposition procedure could be a promising method to develop implantable biosensors with long-time. stability.
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