Generating colorful carrot germplasm through metabolic engineering of betalains pigments

Betalains are tyrosine-derived plant pigments exclusively found in the Caryophyllales order and some higher fungi and generally classified into two groups: red-violet betacyanins and yellow-orange betaxanthins. Betalains attract great scientific and economic interest because of their relatively simple biosynthesis pathway, attractive colors and health-promoting properties. Co-expressing two core genes BvCYP76AD1 and BvDODA1 with or without a glycosyltransferase gene MjcDOPA5GT allowed the engineering of carrot (an important taproot vegetable) to produce a palette of unique colors. The highest total betalains content, 943.2 mu g center dot g(-1) DW, was obtained in carrot taproot transformed with p35S:RUBY which produces all of the necessary enzymes for betalains synthesis. Root-specific production of betalains slightly relieved tyrosine consumption revealing the possible bottleneck in betalains production. Furthermore, a unique volcano-like phenotype in carrot taproot cross-section was created by vascular cambium-specific production of betalains. The betalains-fortified carrot in this study is thus anticipated to be used as functional vegetable and colorful carrot germplasm in breeding to promote health.

Automated summary

Betalains are plant pigments found in plants and fungi, and they have attracted scientific and economic interest due to their simple biosynthesis pathway, attractive colors, and health-promoting properties. The researchers engineered carrots to produce unique colors by co-expressing specific genes. The highest betalains content was achieved in the transgenic carrots, and the production of betalains in a specific tissue created a unique phenotype. The betalains-fortified carrot has potential as a functional vegetable and a valuable germplasm resource for breeding.