Multi-gene phylogeny and morphology of two new Phyllosticta (Phyllostictaceae, Botryosphaeriales) species from China

Phyllosticta (Phyllostictaceae, Botryosphaeriales) includes plant pathogens, endophytes and saprobes, oc-curring on various hosts worldwide. During the present study, isolates associated with leaf spots were ob-tained from the hosts Quercus aliena and Viburnum odoratissimum, and identified based on morphological features and phylogenetic inference from the analyses of five loci (ITS, LSU, tef1, act and gapdh). Results supported the introduction of two novel species, namely Phyllosticta anhuiensis and P. guangdongensis. Phylogenetically, P. anhuiensis and P. guangdongensis formed two well-separated lineages in the P. concentri-ca and P. capitalensis species complexes, distinguishing from all presently accepted species in this genus by DNA sequence data. Morphologically, P. anhuiensis and P. guangdongensis have the typical structure of the genus Phyllosticta, and differed from their closely related species by the length of the conidial appendage.

Automated summary

During this study, two new species of Phyllosticta were identified from hosts Quercus aliena and Viburnum odoratissimum. These new species showed distinct lineages and morphological differences from the currently accepted species in this genus.