Molecular characterization of the Indian isolate (Ka-To) of tomato spotted wilt virus (TSWV) infecting tomato (Solanum lycopersicum L.)

Tomato spotted wilt virus (TSWV) infecting tomato has been identified as an emerging constraint for tomato cultivation in the southern Indian states of Karnataka and Tamil Nadu. Infection of TSWV produces circular necrotic ring spots on leaves, stem and floral necrosis and necrotic ringspots on fruits of tomato. In this study, we describe the characterization of TSWV isolate (Ka-To) infecting tomato from India based on biological, serological and molecular assay. Pathogenicity of TSWV (Ka-To) isolate was established by mechanical inoculation of sap from infected leaves on tomato, cowpea and datura which expressed necrotic or chlorotic local lesions. Samples were tested positive in the serological assay performed with TSWV-specific immunostrips. Further, reverse transcription polymerase chain reaction (RT-PCR) amplification of coat protein gene followed by sequencing, unequivocally confirmed the identity of TSWV. The obtained full-length nucleotide sequences of Ka-To isolate [L RNA-MK977648; M RNA-MK977649; and S RNA-MK977650] had greater similarity to the TSWV isolates of Spain and Hungary infecting tomato and pepper. The phylogenetic and recombination analysis showed the evidence for reassortment and recombination in the genome of Ka-To isolate. To the best of our knowledge, this is the first confirmed evidence for the occurrence of TSWV on tomato in India. Information obtained in this study issues a forewarning on the emergence of TSWV on vegetable ecosystem in the Indian subcontinent, requiring urgent management strategies to curtail its pestilence.

Automated summary

This study characterizes the Tomato spotted wilt virus (TSWV) isolate (Ka-To) infecting tomato from India through biological, serological, and molecular assays. The pathogenicity of TSWV (Ka-To) isolate was confirmed by mechanical inoculation on tomato, cowpea, and datura, resulting in necrotic or chlorotic local lesions. Serological assay and RT-PCR amplification confirmed the identity of TSWV. The obtained nucleotide sequences of Ka-To isolate showed similarity to TSWV isolates from Spain and Hungary, indicating reassortment and recombination in the genome.