Efficient mRNA Delivery with Lyophilized Human Serum Albumin-Based Nanobubbles

In this study, we developed an efficient mRNA delivery vehicle by optimizing a lyophilization method for preserving human serum albumin-based nanobubbles (HSA-NBs), bypassing the need for artificial stabilizers. The morphology of the lyophilized material was verified using scanning electron microscopy, and the concentration, size, and mass of regenerated HSA-NBs were verified using flow cytometry, nanoparticle tracking analysis, and resonance mass measurements, and compared to those before lyophilization. The study also evaluated the response of HSA-NBs to 1 MHz ultrasound irradiation and their ultrasound (US) contrast effect. The functionality of the regenerated HSA-NBs was confirmed by an increased expression of intracellularly transferred Gluc mRNA, with increasing intensity of US irradiation. The results indicated that HSA-NBs retained their structural and functional integrity markedly, post-lyophilization. These findings support the potential of lyophilized HSA-NBs, as efficient imaging, and drug delivery systems for various medical applications.

Automated summary

In this study, an efficient mRNA delivery vehicle was developed by optimizing lyophilization method for preserving human serum albumin-based nanobubbles (HSA-NBs), which eliminated the need for artificial stabilizers. The morphology of the lyophilized material was verified using scanning electron microscopy, and the properties of regenerated HSA-NBs were assessed using flow cytometry, nanoparticle tracking analysis, and resonance mass measurements. The functionality of the regenerated HSA-NBs was confirmed by increased expression of intracellularly transferred mRNA under ultrasound irradiation. The findings indicate the potential of lyophilized HSA-NBs as efficient imaging and drug delivery systems for medical applications.