Genomic Insights into the Origin of a Thermotolerant Tomato Line and Identification of Candidate Genes for Heat Stress

Climate change represents the main problem for agricultural crops, and the constitution of heat-tolerant genotypes is an important breeder's strategy to reduce yield losses. The aim of the present study was to investigate the whole genome of a heat-tolerant tomato genotype (E42), in order to identify candidate genes involved in its response to high temperature. E42 presented a high variability for chromosomes 1, 4, 7 and 12, and phylogenetic analysis highlighted its relationship with the wild S. pimpinellifolium species. Variants with high (18) and moderate (139) impact on protein function were retrieved from two lists of genes related to heat tolerance and reproduction. This analysis permitted us to prioritize a subset of 35 candidate gene mapping in polymorphic regions, some colocalizing in QTLs controlling flowering in tomato. Among these genes, we identified 23 HSPs, one HSF, six involved in flowering and five in pollen activity. Interestingly, one gene coded for a flowering locus T1 and mapping on chromosome 11 resides in a QTL region controlling flowering and also showed 100% identity with an S. pimpinellifolium allele. This study provides useful information on both the E42 genetic background and heat stress response, and further studies will be conducted to validate these genes.

Automated summary

Climate change is a major problem for agricultural crops, and breeding heat-tolerant genotypes is an important strategy to reduce yield losses. In this study, the whole genome of a heat-tolerant tomato genotype (E42) was investigated to identify candidate genes involved in its response to high temperature. Variants with high and moderate impact on protein function were found in genes related to heat tolerance and reproduction. This study provides valuable information on the genetic background and heat stress response of E42.