4.6 Article

Reference Guided De Novo Genome Assembly of Transformation Pliable Solanum lycopersicum cv. Pusa Ruby

Journal

GENES
Volume 14, Issue 3, Pages -

Publisher

MDPI
DOI: 10.3390/genes14030570

Keywords

whole genome sequencing; Solanum lycopersicum; tomato; Pusa Ruby; reference-guided assembly; transformation protocol; Agrobacterium tumefaciens

Ask authors/readers for more resources

We reported a reference-guided genome assembly of the superior tomato cultivar Solanum lycopersicum cv. Pusa Ruby (PR), which covers 97.6% of the single-copy genes in the solanales order. The PR genome contains 34,075 genes and 423,288 variants, with 127,131 intragenic variants and 1232 high impact variants. PR shows the lowest affinity towards Fla.8924 among the compared tomato varieties. The high regeneration rate (45.51%) of PR was analyzed and only AGAMOUS-LIKE15 was found to have a null mutation.
Solanum lycopersicum cv. Pusa Ruby (PR) is a superior tomato cultivar routinely used as a model tomato variety. Here, we report a reference-guided genome assembly for PR, covering 97.6% of the total single-copy genes in the solanales order. The PR genome contains 34,075 genes and 423,288 variants, out of which 127,131 are intragenic and 1232 are of high impact. The assembly was packaged according to PanSol guidelines (N50 = 60,396,827) with the largest scaffold measuring 85 megabases. The similarity of the PR genome assembly to Heinz1706, M82, and Fla.8924 was measured and the results suggest PR has the lowest affinity towards the hybrid Fla.8924. We then analyzed the regeneration efficiency of PR in comparison to another variety, Pusa Early Dwarf (PED). PR was found to have a high regeneration rate (45.51%) and therefore, we performed allele mining for genes associated with regeneration and found that only AGAMOUS-LIKE15 has a null mutation. Further, allele mining for fruit quality-related genes was also executed. The PR genome has an Ovate mutation leading to round fruit shape, causing economically undesirable fruit cracking. This genomic data can be potentially used for large scale crop improvement programs as well as functional annotation studies.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available