4.6 Review

Cellular complexity of the peripheral nervous system: Insights from single-cell resolution

Related references

Note: Only part of the references are listed.
Article Cell & Tissue Engineering

Enteric glial cell heterogeneity regulates intestinal stem cell niches

Meryem B. Baghdadi et al.

Summary: This study investigates the heterogeneity and regulation of enteric glial cells (EGCs) in the intestine during homeostasis and chronic inflammatory bowel disease. A specific subset of glial cells, expressing GFAP, is found to regulate the repair potential of intestinal stem cells (ISCs) through the expression of WNT ligands. The dynamically regulated heterogeneity of EGCs is revealed to be a key part of the intestinal stem cell niche in regeneration and disease.

CELL STEM CELL (2022)

Article Neurosciences

Disentangling glial diversity in peripheral nerves at single-nuclei resolution

Aldrin K. Y. Yim et al.

Summary: Using single-nuclei RNA sequencing, this study analyzed the diversity of cell types in peripheral nerves and identified a novel myelinating Schwann cell subtype that is depleted in ALS cases. These findings serve as a valuable reference for future studies of nerve biology and disease.

NATURE NEUROSCIENCE (2022)

Article Cell Biology

Spatial transcriptomics of dorsal root ganglia identifies molecular signatures of human nociceptors

Diana Tavares-Ferreira et al.

Summary: Nociceptors, specialized sensory neurons, play a crucial role in detecting and generating pain signals. This study used spatial transcriptomics to characterize the transcriptomes of human nociceptors, identifying different clusters and potential drug targets in the human dorsal root ganglia (DRG). Comparison with mouse sensory neuron transcriptomes and nonhuman primate DRG neuronal subtypes revealed conserved gene expression patterns but also species-specific differences. Sex differences in DRG subpopulation transcriptomes were also identified. This comprehensive spatial characterization of human nociceptors has significant implications for the development of improved treatments for pain disorders.

SCIENCE TRANSLATIONAL MEDICINE (2022)

Article Cell Biology

Single-cell analyses reveal the differentiation shifts of Schwann cells in neonatal rat sciatic nerves

Ruirui Zhang et al.

Summary: The study analyzed single-cell transcriptional profiling of Schwann cells in neonatal rat sciatic nerves, revealing cellular heterogeneity and dynamic changes. It also identified differentiation trajectory from certain Schwann cell subtypes to others, with functional interpretation showing genetic signatures of DNA replication and acquisition of mesenchymal traits in subtype 3 Schwann cells.

JOURNAL OF CELLULAR PHYSIOLOGY (2022)

Article Biochemistry & Molecular Biology

The metabolomic profiling identifies N, N-dimethylglycine as a facilitator of dorsal root ganglia neuron axon regeneration after injury

Junjie Zhang et al.

Summary: Identifying novel molecules involved in axon regeneration in the peripheral nervous system (PNS) can help in developing therapeutic strategies for repairing axon damage in both PNS and CNS. Metabolism and axon regeneration are closely linked, with several metabolic pathways being significantly altered during the process. N, N-dimethylglycine (DMG) was found to have a promoting effect on regenerative ability post injury, suggesting it as a potential candidate drug for nerve repair.

FASEB JOURNAL (2022)

Article Neurosciences

Single-cell transcriptome analysis of regenerating RGCs reveals potent glaucoma neural repair genes

Liang Li et al.

Summary: Axon regeneration in CNS axonopathies, such as glaucoma, holds great potential for neural repair. In this study, a strategy was developed to specifically label and purify regenerating retinal ganglion cells (RGCs) and non-regenerating RGCs from the same mice. Transcriptome analysis revealed novel genes, including Anxa2, that significantly promote axon regeneration. Anxa2, along with its downstream effectors ILK and Mpp1, were found to protect RGCs and axons, preserving visual function in a glaucoma model. This innovative strategy shows exciting potential for identifying effective candidates for neural repair.

NEURON (2022)

Article Neurosciences

Cell populations in neonatal rat peripheral nerves identified by single-cell transcriptomics

Ruirui Zhang et al.

Summary: The study used high-coverage single-cell sequencing to reveal the cell diversity and heterogeneity of Schwann cells in the peripheral nerves of neonatal rats. A total of 10 cell types were identified in SN and DRG with distinct cell-cell communication patterns.
Article Gastroenterology & Hepatology

scRNA-Seq Reveals New Enteric Nervous System Roles for GDNF, NRTN, and TBX3

Christina M. Wright et al.

Summary: Single cell analyses identified genes differentially expressed in myenteric neuron subtypes and revealed new roles for TBX3, GDNF, and NRTN in enteric neuron activity. These findings can help advance molecular diagnostic studies and develop novel therapeutics for bowel motility disorders.

CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY (2021)

Article Cell Biology

Single-cell transcriptomic analysis of somatosensory neurons uncovers temporal development of neuropathic pain

Kaikai Wang et al.

Summary: Researchers identified new neuronal clusters induced by spared nerve injury (SNI) through single-cell RNA sequencing on dissociated mouse DRG cells, revealing the transcriptomic changes during the process. Additionally, activation of specific transcription factors within 2 days after nerve injury might lead to changes in gene expression. This study provides a new molecular mechanism basis for understanding the development of neuropathic pain.

CELL RESEARCH (2021)

Article Multidisciplinary Sciences

A multimodal cell census and atlas of the mammalian primary motor cortex

Edward M. Callaway et al.

Summary: This study presents a multimodal cell census and atlas of the mammalian primary motor cortex, integrating various molecular and spatial information to reveal a unified genetic landscape of cortical cell types. The results establish a mechanistic framework of neuronal cell-type organization by linking molecular genetic information with phenotypic properties.

NATURE (2021)

Article Neurosciences

Interneuron origin and molecular diversity in the human fetal brain

Yuan Yu et al.

Summary: The authors used single-cell RNA sequencing and in situ sequencing to profile the interneuron origin and molecular diversity in the human fetal brain, demonstrating the complexity and logic behind the specification of diverse interneurons in humans.

NATURE NEUROSCIENCE (2021)

Article Multidisciplinary Sciences

Single cell transcriptomics of primate sensory neurons identifies cell types associated with chronic pain

Jussi Kupari et al.

Summary: This study classified non-human primate dorsal root ganglion sensory neurons based on their transcriptome and mapped human pain heritability to neuronal types. The findings revealed the cellular origin of chronic pain by showing genome-wide associations converging on two different neuronal types with different genetic susceptibilities for chronic pain.

NATURE COMMUNICATIONS (2021)

Article Multidisciplinary Sciences

Joint single-cell multiomic analysis in Wnt3a induced asymmetric stem cell division

Zhongxing Sun et al.

Summary: This study developed a method to jointly profile epigenome and transcriptome in the same cell, providing insights into the gene regulatory programs involved in maintaining and resetting stem cell fate under localized Wnt3a signaling.

NATURE COMMUNICATIONS (2021)

Article Genetics & Heredity

Direct Comparative Analyses of 10X Genomics Chromium and Smart-seq2

Xiliang Wang et al.

Summary: This study systematically evaluated the features and limitations of two frequently used single-cell RNA sequencing platforms, 10X Genomics Chromium and Smart-seq2, by comparing their data generated from the same CD45(-) cell samples. Smart-seq2 detected more genes in a cell, especially low abundance and alternatively spliced transcripts, while 10X-based data showed more severe dropout problems but could detect rare cell types. The study provides insights into the characteristics of these technologies and their applications in single-cell transcriptomics.

GENOMICS PROTEOMICS & BIOINFORMATICS (2021)

Article Cell Biology

Patch-seq of mouse DRG neurons reveals candidate genes for specific mechanosensory functions

Thibaud Parpaite et al.

Summary: This study combines recordings of mechanosensitive currents in mechanosensory neurons with single-cell RNA sequencing to explore the role of transcriptional profiles on mechanotransduction mechanisms. The research revealed that Piezo2 is enriched in rapidly adapting neurons expressing MS currents, while Tmem120a and Tmem150c are uniformly expressed in all mechanosensory neuron subtypes.

CELL REPORTS (2021)

Article Biology

Profiling sensory neuron microenvironment after peripheral and central axon injury reveals key pathways for neural repair

Oshri Avraham et al.

Summary: Peripheral nerve injuries lead to the appearance of a distinct subset of satellite glial cells in DRG and activation of the PPAR alpha signaling pathway, promoting axon regeneration. Central axon injuries do not trigger the same response, highlighting the potential for manipulating non-neuronal cells to promote functional recovery after CNS injuries or disease.

ELIFE (2021)

Article Biology

Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons

Minh Q. Nguyen et al.

Summary: Through single nuclear transcriptomics of human DRG neurons, it was found that different types of neurons share similarities in their transcriptomic features, but there are species differences in the expression of genes related to sensory function. Surprisingly, several transcriptomic classes without clear equivalents in other species were identified.

ELIFE (2021)

Article Biology

Transcriptional profiling of mouse peripheral nerves to the single-cell level to build a sciatic nerve ATlas (SNAT)

Daniel Gerber et al.

Summary: Peripheral nerves are complex structures composed of diverse cell types, including Schwann cells, endothelial cells, immune system-associated cells, and connective tissue-resident cells. By establishing transcriptional profiles and utilizing RNA sequencing techniques, researchers have gained insights into the cellular composition and development of the mouse sciatic nerve. The results have been integrated into the Sciatic Nerve ATlas (SNAT) resource, providing a valuable reference point for future studies.

ELIFE (2021)

Article Cell Biology

Diversity of developing peripheral glia revealed by single-cell RNA sequencing

Ozge E. Tasdemir-Yilmaz et al.

Summary: The study identified differences in transcriptional profiles of glial precursors in somatosensory and auditory systems, but they ultimately undergo convergent differentiation to form molecularly uniform Schwann cells. However, satellite glial cells in somatosensory and auditory systems retain system-specific features.

DEVELOPMENTAL CELL (2021)

Article Multidisciplinary Sciences

The emergence of transcriptional identity in somatosensory neurons

Nikhil Sharma et al.

NATURE (2020)

Article Multidisciplinary Sciences

Redefining the heterogeneity of peripheral nerve cells in health and autoimmunity

Jolien Wolbert et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

Article Multidisciplinary Sciences

Paracrine orchestration of intestinal tumorigenesis by a mesenchymal niche

Manolis Roulis et al.

NATURE (2020)

Article Biochemistry & Molecular Biology

The Human and Mouse Enteric Nervous System at Single-Cell Resolution

Eugene Drokhlyansky et al.

Article Multidisciplinary Sciences

Frataxin gene editing rescues Friedreich's ataxia pathology in dorsal root ganglia organoid-derived sensory neurons

Pietro Giuseppe Mazzara et al.

NATURE COMMUNICATIONS (2020)

Article Multidisciplinary Sciences

Satellite glial cells promote regenerative growth in sensory neurons

Oshri Avraham et al.

NATURE COMMUNICATIONS (2020)

Article Cell & Tissue Engineering

Mesenchymal Precursor Cells in Adult Nerves Contribute to Mammalian Tissue Repair and Regeneration

Matthew J. Carr et al.

CELL STEM CELL (2019)

Review Biochemistry & Molecular Biology

Single-cell RNA sequencing: Technical advancements and biological applications

Eva Hedlund et al.

MOLECULAR ASPECTS OF MEDICINE (2018)

Article Biochemistry & Molecular Biology

Molecular Architecture of the Mouse Nervous System

Amit Zeisel et al.

Article Biochemistry & Molecular Biology

Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease

James Kinchen et al.

Article Multidisciplinary Sciences

Developmental diversification of cortical inhibitory interneurons

Christian Mayer et al.

NATURE (2018)

Review Cell Biology

Single Cell Multi-Omics Technology: Methodology and Application

Youjin Hu et al.

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY (2018)

Review Neurosciences

Enteric glia: the most alimentary of all glia

Vladimir Grubisic et al.

JOURNAL OF PHYSIOLOGY-LONDON (2017)

Article Biochemistry & Molecular Biology

Single-cell RNA-sequencing: The future of genome biology is now

Simone Picelli

RNA BIOLOGY (2017)

Letter Biochemical Research Methods

Single-cell sequencing reveals dissociation-induced gene expression in tissue subpopulations

Susanne C. van den Brink et al.

NATURE METHODS (2017)

Article Neurosciences

WHOLE TRANSCRIPTOME EXPRESSION OF TRIGEMINAL GANGLIA COMPARED TO DORSAL ROOT GANGLIA IN RATTUS NORVEGICUS

Lisette Johanna Antonia Kogelman et al.

NEUROSCIENCE (2017)

Article Neurosciences

The Molecular Fingerprint of Dorsal Root and Trigeminal Ganglion Neurons

Douglas M. Lopes et al.

FRONTIERS IN MOLECULAR NEUROSCIENCE (2017)

Article Multidisciplinary Sciences

Diversity amongst trigeminal neurons revealed by high throughput single cell sequencing

Minh Q. Nguyen et al.

PLOS ONE (2017)

Review Neurosciences

Specification, plasticity and evolutionary origin of peripheral glial cells

Maria Eleni Kastriti et al.

CURRENT OPINION IN NEUROBIOLOGY (2017)

Review Neurosciences

Intrinsic Control of Axon Regeneration

Zhigang He et al.

NEURON (2016)

Article Multidisciplinary Sciences

Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling

Susanne Nichterwitz et al.

NATURE COMMUNICATIONS (2016)

Article Multidisciplinary Sciences

Single-cell RNA-seq reveals distinct injury responses in different types of DRG sensory neurons

Ganlu Hu et al.

SCIENTIFIC REPORTS (2016)

Article Biochemistry & Molecular Biology

Droplet Barcoding for Single-Cell Transcriptomics Applied to Embryonic Stem Cells

Allon M. Klein et al.

Article Biochemistry & Molecular Biology

Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets

Evan Z. Macosko et al.

Article Multidisciplinary Sciences

RNA-Seq Analysis of Human Trigeminal and Dorsal Root Ganglia with a Focus on Chemoreceptors

Caroline Flegel et al.

PLOS ONE (2015)

Article Multidisciplinary Sciences

Combinatorial labeling of single cells for gene expression cytometry

H. Christina Fan et al.

SCIENCE (2015)

Article Cell Biology

Schwann Cells: Development and Role in Nerve Repair

Kristjan R. Jessen et al.

COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY (2015)

Article Neurosciences

Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing

Dmitry Usoskin et al.

NATURE NEUROSCIENCE (2015)

Article Neurosciences

HDAC1 and HDAC2 Control the Specification of Neural Crest Cells into Peripheral Glia

Claire Jacob et al.

JOURNAL OF NEUROSCIENCE (2014)

Article Biochemical Research Methods

Full-length RNA-seq from single cells using Smart-seq2

Simone Picelli et al.

NATURE PROTOCOLS (2014)

Review Gastroenterology & Hepatology

Enteric nervous system development: migration, differentiation, and disease

Jonathan I. Lake et al.

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY (2013)

Article Biochemical Research Methods

Smart-seq2 for sensitive full-length transcriptome profiling in single cells

Simone Picelli et al.

NATURE METHODS (2013)

Article Biotechnology & Applied Microbiology

Full-length mRNA-Seq from single-cell levels of RNA and individual circulating tumor cells

Daniel Ramskold et al.

NATURE BIOTECHNOLOGY (2012)

Article Cell Biology

CEL-Seq: Single-Cell RNA-Seq by Multiplexed Linear Amplification

Tamar Hashimshony et al.

CELL REPORTS (2012)

Article Biochemistry & Molecular Biology

Characterization of the single-cell transcriptional landscape by highly multiplex RNA-seq

Saiful Islam et al.

GENOME RESEARCH (2011)

Review Medicine, Research & Experimental

Labeled lines meet and talk: population coding of somatic sensations

Qiufu Ma

JOURNAL OF CLINICAL INVESTIGATION (2010)

Article Biochemical Research Methods

mRNA-Seq whole-transcriptome analysis of a single cell

Fuchou Tang et al.

NATURE METHODS (2009)

Article Biochemistry & Molecular Biology

Glial cells

KR Jessen

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY (2004)