An electrochemical sensor based on graphene-chitosan-cyclodextrin modification for the detection of Staphylococcus aureus

This study reports the synthesis of a novel graphene/chitosan/beta-cyclodextrin composite material (GO/CS/beta-CD) via a one-step chemical reduction method, which combines the advantages of graphene, chitosan, and beta-cyclodextrin. The morphology and structure of the composite were characterized using various techniques, such as scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and Raman spectroscopy. Subsequently, sortase A (SA) was immobilized onto the GO/CS/beta-CD for the detection of Staphylococcus aureus. The sensor exhibited a good linear relationship within the concentration range of 30-300 CFU/mL, with a detection limit of 12 CFU/mL. The GO/CS/beta-CD composite material showed enhanced properties due to the synergistic effect of graphene, chitosan, and beta-cyclodextrin. The immobilization of sortase A onto the composite material improved the sensitivity and selectivity of the sensor for the detection of S. aureus. This study presents a novel graphene/chitosan/beta-cyclodextrin composite material with immobilized sortase A, demonstrating enhanced sensitivity and selectivity for the detection of Staphylococcus aureus, which has potential for the development of high-performance sensors in various fields.

Automated summary

This study successfully synthesized a novel graphene/chitosan/beta-cyclodextrin composite material (GO/CS/beta-CD) via a one-step chemical reduction method, combining the advantages of graphene, chitosan, and beta-cyclodextrin. The composite was characterized using techniques such as scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and Raman spectroscopy. Sortase A (SA) was immobilized onto the GO/CS/beta-CD for the detection of Staphylococcus aureus, showing a good linear relationship within the concentration range of 30-300 CFU/mL and a detection limit of 12 CFU/mL. The GO/CS/beta-CD composite material, with immobilized sortase A, demonstrated enhanced sensitivity and selectivity for the detection of Staphylococcus aureus.