Comparative Study of UV Radiation Resistance and Reactivation Characteristics of E. coli ATCC 8739 and Native Strains: Implications for Water Disinfection

In certain countries where fresh water is in short supply, the effluents from wastewater treatment plants are being recycled for other uses. For quality assurance, tertiary disinfection treatments are required. This study aims to evaluate the inactivating efficacy with an ultraviolet (UV) system on fecal bacteria from effluents of urban wastewater treatment facilities and the post-treatment influence of the environmental illumination. The effect from different UV doses was determined for native and standardized lyophilized strains of Escherichia coli right after the irradiation as well as after 24 h of incubation under light or dark conditions. To achieve 3 log-reductions of the initial bacterial concentration, a UV dose of approximately 12 mJ cm(-2) is needed for E. coli ATCC 8739 and native E. coli. However, there is a risk of the reactivation of 0.19% and 1.54% of the inactivated organisms, respectively, if the treated organisms are stored in an illuminated environment. This suggests that the post-treatment circumstances affect the treatment success; storing the treated water under an illuminated environment may pose a risk even if an effective inactivation was achieved during the irradiation.

Automated summary

In countries with water scarcity, wastewater from treatment plants is being recycled. To ensure quality, tertiary disinfection treatments are required. This study evaluates the efficacy of an ultraviolet (UV) system in inactivating fecal bacteria in urban wastewater effluents and the post-treatment influence of environmental illumination. The results show that a UV dose of approximately 12 mJ cm(-2) is needed to achieve a 3 log-reduction of Escherichia coli, but there is a risk of reactivation if the treated organisms are stored in an illuminated environment.