☆ 4.7 Review

Current and prospective strategies for advancing the targeted delivery of CRISPR/Cas system via extracellular vesicles

JOURNAL OF NANOBIOTECHNOLOGY (2023)

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Exosomes and biomimetic nanovesicles-mediated anti-glioblastoma therapy: A head-to-head comparison

Jun-Yong Wu et al.

Summary: The study compared Exos and BNVs for brain tumor drug delivery, finding that BNVs derived from brain-derived endothelial cells are a promising alternative with higher yield and similar drug-loading capacity to natural exosomes. Despite different mechanisms, BNV/DOX showed similar therapeutic effects to Exo/DOX in inhibiting the progression of glioblastoma, with minimal systemic toxicity. The findings suggest that autologous BNVs are an effective alternative to Exos for brain tumor nanomedicine.

JOURNAL OF CONTROLLED RELEASE (2021)

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Article Medicine, Research & Experimental

Macropinocytosis-Inducible Extracellular Vesicles Modified with Antimicrobial Protein CAP18-Derived Cell-Penetrating Peptides for Efficient Intracellular Delivery

Kosuke Noguchi et al.

Summary: The antimicrobial protein CAP18 and its derived peptides have macropinocytosis-inducible functions and high potential for use as extracellular vesicle-based drug delivery carriers. Modification of these peptides with a hydrophobic moiety allows easy introduction onto EV membranes for enhanced cellular uptake. The glycosaminoglycan-dependent induction of macropinocytosis by the peptide modification efficiently enables cytosolic delivery of biologically functional proteins encapsulated in EVs, demonstrating a useful technique for intracellular delivery of biofunctional molecules.

MOLECULAR PHARMACEUTICS (2021)

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Review Biochemical Research Methods

The power of imaging to understand extracellular vesicle biology in vivo

Frederik J. Verweij et al.

Summary: Extracellular vesicles (EVs) are nano-sized lipid bilayer vesicles released by virtually every cell type, with diverse biological activities. Recent advances in live and high-resolution microscopy techniques, combined with innovative EV labeling strategies, provide new tools to study EVs in vivo at the single-vesicle level.

NATURE METHODS (2021)

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Review Nanoscience & Nanotechnology

Extracellular vesicles as a next-generation drug delivery platform

Inge Katrin Herrmann et al.

Summary: Extracellular vesicles play a crucial role in cell-to-cell communication and can serve as drug carriers with advantages over synthetic carriers. However, clinical translation of extracellular vesicle-based therapies remains challenging, requiring further research and development efforts.

NATURE NANOTECHNOLOGY (2021)

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Article Cell Biology

Targeted delivery of CRISPR-Cas9 and transgenes enables complex immune cell engineering

Jennifer R. Hamilton et al.

Summary: A new strategy has been developed in this study, which uses engineered lentiviral particles to introduce Cas9 RNPs and a CAR transgene into human T cells ex vivo, allowing targeting of specific cell types within a mixed population. This approach provides a method for genetic modification of immune cells without the need for electroporation.

CELL REPORTS (2021)

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Review Biotechnology & Applied Microbiology

Artificial exosomes for translational nanomedicine

Yong-Jiang Li et al.

Summary: Artificial exosomes, produced through nanobiotechnology, have emerged as effective drug delivery carriers and include various types such as nanovesicles, exosome-mimetic, and hybrid exosomes. These artificial exosomes offer solutions to overcome limitations of natural exosomes and are considered powerful alternatives for drug delivery.

JOURNAL OF NANOBIOTECHNOLOGY (2021)

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Article Biotechnology & Applied Microbiology

CRISPR/Cas9-induced β-carotene hydroxylase mutation in Dunaliella salina CCAP19/18

Lina Hu et al.

Summary: This study successfully performed targeted gene editing on D. saline using CRISPR/Cas system, significantly increasing the levels of beta-carotene in mutants. These results have important practical implications for increasing carotene yield and meeting industry demand.

AMB EXPRESS (2021)

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Article Cell Biology

An engineered CD81-based combinatorial library for selecting recombinant binders to cell surface proteins: Laminin binding CD81 enhances cellular uptake of extracellular vesicles

Stefan Vogt et al.

Summary: Research has shown that by using the CD81 protein to recognize target antigens, the targeted delivery capability of EVs can be enhanced, and this has been validated in mammalian cells. Mutant sequences are reformatted into full-length CD81 and expressed in EVs produced by HeLa cells, showing improved uptake by specific antigens.

JOURNAL OF EXTRACELLULAR VESICLES (2021)

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Review Biochemistry & Molecular Biology

Spatiotemporal control of CRISPR/Cas9 gene editing

Chenya Zhuo et al.

Summary: The CRISPR/Cas9 gene editing technology shows promise in treating genetic and infectious diseases but faces challenges in precise editing. Emerging strategies to regulate CRISPR/Cas9 activity have shown potential in improving spatiotemporal control, but obstacles and limitations remain for clinical translation. Further exploration is needed to enhance the spatiotemporal operability of CRISPR/Cas9.

SIGNAL TRANSDUCTION AND TARGETED THERAPY (2021)

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Article Medicine, Research & Experimental

Targeted delivery of neural progenitor cell-derived extracellular vesicles for anti-inflammation after cerebral ischemia

Tian Tian et al.

Summary: Ischemic stroke is a major cause of death and anti-inflammatory strategies are promising for reducing brain injury during reperfusion. Stem cell-derived extracellular vesicles (EVs) have emerged as therapeutic agents for immune modulation. By attaching targeting ligands onto EVs using a recombinant fusion protein, researchers were able to deliver anti-inflammatory EVs to the ischemic brain, showing promising results in suppressing inflammation.

THERANOSTICS (2021)

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Article Pharmacology & Pharmacy

Preserving extracellular vesicles for biomedical applications: consideration of storage stability before and after isolation

Fumin Yuan et al.

Summary: Extracellular vesicles (EVs) are nanovesicles released by various cell types, known for their potent biological activities. While -80 degrees C is considered as the most favorable condition for storage, challenges remain in understanding the impact of storage conditions on EVs, indicating further research is needed.

DRUG DELIVERY (2021)

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Article Pharmacology & Pharmacy

Engineered targeting tLyp-1 exosomes as gene therapy vectors for efficient delivery of siRNA into lung cancer cells

Jing Bai et al.

ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES (2020)

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Article Biotechnology & Applied Microbiology

Genetically Engineered Cell-Derived Nanoparticles for Targeted Breast Cancer Immunotherapy

Xiaojing Shi et al.

MOLECULAR THERAPY (2020)

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Article Cell Biology

Blood concentrations of small extracellular vesicles are determined by a balance between abundant secretion and rapid clearance

Akihiro Matsumoto et al.

JOURNAL OF EXTRACELLULAR VESICLES (2020)

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Article Chemistry, Multidisciplinary

Responsive Exosome Nano-bioconjugates for Synergistic Cancer Therapy

Weidong Nie et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

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Article Engineering, Biomedical

Ultrasound-activated particles as CRISPR/Cas9 delivery system for androgenic alopecia therapy

Jee-Yeon Ryu et al.

BIOMATERIALS (2020)

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Article Engineering, Biomedical

Mesenchymal stem cell-derived magnetic extracellular nanovesicles for targeting and treatment of ischemic stroke

Han Young Kim et al.

BIOMATERIALS (2020)

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Review Chemistry, Multidisciplinary

Receptor specific, stimuli responsive and subcellular targeted approaches for effective therapy of Alzheimer: Role of surface engineered nanocarriers

Divya Gopalan et al.

JOURNAL OF CONTROLLED RELEASE (2020)

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Review Multidisciplinary Sciences

The promise and challenge of therapeutic genome editing

Jennifer A. Doudna

NATURE (2020)

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Article Multidisciplinary Sciences

CRISPR-engineered T cells in patients with refractory cancer

Edward A. Stadtmauer et al.

SCIENCE (2020)

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Article Engineering, Biomedical

Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation

Zhaogang Yang et al.

NATURE BIOMEDICAL ENGINEERING (2020)

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Article Biochemical Research Methods

In Situ One-Step Fluorescence Labeling Strategy of Exosomes via Bioorthogonal Click Chemistry for Real-Time Exosome Tracking In Vitro and In Vivo

Sukyung Song et al.

BIOCONJUGATE CHEMISTRY (2020)

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Article Biotechnology & Applied Microbiology

Preclinical development of a novel CD47 nanobody with less toxicity and enhanced anti-cancer therapeutic potential

Linlin Ma et al.

JOURNAL OF NANOBIOTECHNOLOGY (2020)

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Article Biotechnology & Applied Microbiology

Engineered exosomes for targeted co-delivery of miR-21 inhibitor and chemotherapeutics to reverse drug resistance in colon cancer

Gaofeng Liang et al.

JOURNAL OF NANOBIOTECHNOLOGY (2020)

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Article Multidisciplinary Sciences

Cas9/gRNA-mediated genome editing of yeast mitochondria and Chlamydomonas chloroplasts

Byung-Chun Yoo et al.

PEERJ (2020)

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Review Immunology

The CD47-SIRPα Immune Checkpoint

Meike E. W. Logtenberg et al.

IMMUNITY (2020)

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Article Multidisciplinary Sciences

Alarmin-painted exosomes elicit persistent antitumor immunity in large established tumors in mice

Bingfeng Zuo et al.

NATURE COMMUNICATIONS (2020)

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Article Multidisciplinary Sciences

Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping

Peter Gee et al.

NATURE COMMUNICATIONS (2020)

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Review Cardiac & Cardiovascular Systems

Native and bioengineered extracellular vesicles for cardiovascular therapeutics

Ricardo Cerqueira de Abreu et al.

NATURE REVIEWS CARDIOLOGY (2020)

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Review Cell Biology

RNA delivery by extracellular vesicles in mammalian cells and its applications

Killian O'Brien et al.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2020)

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Article Medicine, Research & Experimental

Ultrasound Assisted Exosomal Delivery of Tissue Responsive mRNA for Enhanced Efficacy and Minimized Off-Target Effects

Wenqi Sun et al.

MOLECULAR THERAPY-NUCLEIC ACIDS (2020)

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Article Biochemistry & Molecular Biology

Targeted Delivery of Mesenchymal Stem Cell-Derived Nanovesicles for Spinal Cord Injury Treatment

Ju-Ro Lee et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

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Article Multidisciplinary Sciences

Codelivery of CRISPR-Cas9 and chlorin e6 for spatially controlled tumor-specific gene editing with synergistic drug effects

Shaohui Deng et al.

SCIENCE ADVANCES (2020)

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Review Pharmacology & Pharmacy

Exosomes and GPI-anchored proteins: Judicious pairs for investigating biomarkers from body fluids

Michel Vidal

ADVANCED DRUG DELIVERY REVIEWS (2020)

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Review Biotechnology & Applied Microbiology

CRISPR/Cas technology promotes the various application ofDunaliella salinasystem

Shuying Feng et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2020)

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Article Engineering, Biomedical

Self-assembly of stem cell membrane-camouflaged nanocomplex for microRNA-mediated repair of myocardial infarction injury

Chi Yao et al.

BIOMATERIALS (2020)

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Review Cell Biology

Golgi Apparatus: An Emerging Platform for Innate Immunity

Ye Tao et al.

TRENDS IN CELL BIOLOGY (2020)

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Article Biochemistry & Molecular Biology

Identification of Lymphatic and Hematogenous Routes of Rapidly Labeled Radioactive and Fluorescent Exosomes through Highly Sensitive Multimodal Imaging

Kyung Oh Jung et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

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Review Biochemistry & Molecular Biology

Gene Editing by Extracellular Vesicles

Dmitry Kostyushev et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

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Article Chemistry, Multidisciplinary

Tropism-facilitated delivery of CRISPR/Cas9 system with chimeric antigen receptor-extracellular vesicles against B-cell malignancies

Qian Xu et al.

JOURNAL OF CONTROLLED RELEASE (2020)

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Article Biochemistry & Molecular Biology

Extracellular vesicles engineered with valency-controlled DNA nanostructures deliver CRISPR/Cas9 system for gene therapy

Jialang Zhuang et al.

NUCLEIC ACIDS RESEARCH (2020)

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Article Cell Biology

Programmable Extracellular Vesicles for Macromolecule Delivery and Genome Modifications

Xiaojuan Zhang et al.

DEVELOPMENTAL CELL (2020)

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Article Nanoscience & Nanotechnology

Improving Tumor Targeting of Exosomal Membrane-Coated Polymeric Nanoparticles by Conjugation with Aptamers

Ziwei Han et al.

ACS APPLIED BIO MATERIALS (2020)

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Article Cell Biology

Fusion protein engineered exosomes for targeted degradation of specific RNAs in lysosomes: a proof-of-concept study

Zhelong Li et al.

JOURNAL OF EXTRACELLULAR VESICLES (2020)

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Article Materials Science, Biomaterials

An engineered exosome for delivering sgRNA:Cas9 ribonucleoprotein complex and genome editing in recipient cells

Yangyang Ye et al.

BIOMATERIALS SCIENCE (2020)

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Article Cell Biology

Design of experiment (DoE)-driven in vitro and in vivo uptake studies of exosomes for pancreatic cancer delivery enabled by copper-free click chemistry-based labelling

Lizhou Xu et al.

JOURNAL OF EXTRACELLULAR VESICLES (2020)

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Article Chemistry, Multidisciplinary

Sequential deletion of CD63 identifies topologically distinct scaffolds for surface engineering of exosomes in living human cells

Natalie Curley et al.

NANOSCALE (2020)

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Review Biotechnology & Applied Microbiology

The application of the CRISPR-Cas9 genome editing machinery in food and agricultural science: Current status, future perspectives, and associated challenges

Ismail Es et al.

BIOTECHNOLOGY ADVANCES (2019)

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Review Biotechnology & Applied Microbiology

Advances in CRISPR-Cas systems for RNA targeting, tracking and editing

Fei Wang et al.

BIOTECHNOLOGY ADVANCES (2019)

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Article Cell & Tissue Engineering

Targeted Disruption of HLA Genes via CRISPR-Cas9 Generates iPSCs with Enhanced Immune Compatibility

Huaigeng Xu et al.

CELL STEM CELL (2019)

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Article Biotechnology & Applied Microbiology

Exosome origin determines cell targeting and the transfer of therapeutic nanoparticles towards target cells

Maria Sancho-Albero et al.

JOURNAL OF NANOBIOTECHNOLOGY (2019)

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Article Chemistry, Multidisciplinary

Embryonic Stem Cells-Derived Exosomes Endowed with Targeting Properties as Chemotherapeutics Delivery Vehicles for Glioblastoma Therapy

Qingwei Zhu et al.

ADVANCED SCIENCE (2019)

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Article Multidisciplinary Sciences

HIV-1 Vpr counteracts HLTF-mediated restriction of HIV-1 infection in T cells

Junpeng Yan et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2019)

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Article Engineering, Biomedical

Cell-free synthesis of connexin 43-integrated exosome-mimetic nanoparticles for siRNA delivery

Mei Lu et al.

ACTA BIOMATERIALIA (2019)

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Review Materials Science, Multidisciplinary

PEGylated liposomes: immunological responses

Marwa Mohamed et al.

SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS (2019)

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Article Chemistry, Multidisciplinary

Friend or Foe? Evidence Indicates Endogenous Exosomes Can Deliver Functional gRNA and Cas9 Protein

Ran Chen et al.

SMALL (2019)

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Article Biotechnology & Applied Microbiology

Exosome-mimetic nanoplatforms for targeted cancer drug delivery

Abi J. Vazquez-Rios et al.

JOURNAL OF NANOBIOTECHNOLOGY (2019)

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Article Cell Biology

Bioengineering commensal bacteria-derived outer membrane vesicles fordelivery of biologics to the gastrointestinal and respiratory tract

Ana L. Carvalho et al.

JOURNAL OF EXTRACELLULAR VESICLES (2019)

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Article Oncology

Microvesicle-Mediated Delivery of Minicircle DNA Results in Effective Gene-Directed Enzyme Prodrug Cancer Therapy

Masamitsu Kanada et al.

MOLECULAR CANCER THERAPEUTICS (2019)

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Letter Medicine, Research & Experimental

Activation of Necroptosis by Engineered Self Tumor-Derived Exosomes Loaded with CRISPR/Cas9

Diana Gulei et al.

MOLECULAR THERAPY-NUCLEIC ACIDS (2019)

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Article Oncology

Glycan-Modified Apoptotic Melanoma-Derived Extracellular Vesicles as Antigen Source for Anti-Tumor Vaccination

Sophie K. Horrevorts et al.

CANCERS (2019)

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Article Cell Biology

Glycan modification of glioblastoma-derived extracellular vesicles enhances receptor-mediated targeting of dendritic cells

Sophie A. Dusoswa et al.

JOURNAL OF EXTRACELLULAR VESICLES (2019)

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Article Biotechnology & Applied Microbiology

Endosomal escape enhancing compounds facilitate functional delivery of extracellular vesicle cargo

Nikki Heath et al.

NANOMEDICINE (2019)

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Article Multidisciplinary Sciences

Search-and-replace genome editing without double-strand breaks or donor DNA

Andrew V. Anzalone et al.

NATURE (2019)

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Review Nanoscience & Nanotechnology

Engineered materials for in vivo delivery of genome-editing machinery

Sheng Tong et al.

NATURE REVIEWS MATERIALS (2019)

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Article Biotechnology & Applied Microbiology

Gesicle-Mediated Delivery of CRISPR/Cas9 Ribonucleoprotein Complex for Inactivating the HIV Provirus

Lee A. Campbell et al.

MOLECULAR THERAPY (2019)

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Article Chemistry, Multidisciplinary

In Vitro and in Vivo RNA Inhibition by CD9-HuR Functionalized Exosomes Encapsulated with miRNA or CRISPR/dCas9

Zhelong Li et al.

NANO LETTERS (2019)

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Article Multidisciplinary Sciences

Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins

Philippe E. Mangeot et al.

NATURE COMMUNICATIONS (2019)

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Letter Cell Biology

In vitro transcribed sgRNA causes cell death by inducing interferon release

Wei Mu et al.

PROTEIN & CELL (2019)

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Article Chemistry, Multidisciplinary

Artificial chimeric exosomes for anti-phagocytosis and targeted cancer therapy

Kai-Long Zhang et al.

CHEMICAL SCIENCE (2019)

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Article Chemistry, Multidisciplinary

Modification of the glycosylation of extracellular vesicles alters their biodistribution in mice

Felix Royo et al.

NANOSCALE (2019)

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Article Chemistry, Multidisciplinary

Aptamer-functionalized exosomes from bone marrow stromal cells target bone to promote bone regeneration

Zhong-Wei Luo et al.

NANOSCALE (2019)

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Article Medicine, Research & Experimental

Focused ultrasound-augmented targeting delivery of nanosonosensitizers from homogenous exosomes for enhanced sonodynamic cancer therapy

Yichen Liu et al.

THERANOSTICS (2019)

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Article Chemistry, Multidisciplinary

Endosomal Escape and Delivery of CRISPR/Cas9 Genome Editing Machinery Enabled by Nanoscale Zeolitic Imidazolate Framework

Shahad K. Alsaiari et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2018)

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Article Engineering, Biomedical

Surface functionalized exosomes as targeted drug delivery vehicles for cerebral ischemia therapy

Tian Tian et al.

BIOMATERIALS (2018)

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Article Nanoscience & Nanotechnology

Engineered exosome-mediated delivery of functionally active miR-26a and its enhanced suppression effect in HepG2 cells

Gaofeng Liang et al.

INTERNATIONAL JOURNAL OF NANOMEDICINE (2018)

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Article Oncology

Anti-HER2 scFv-Directed Extracellular Vesicle-Mediated mRNA-Based Gene Delivery Inhibits Growth of HER2-Positive Human Breast Tumor Xenografts by Prodrug Activation

Jing-Hung Wang et al.

MOLECULAR CANCER THERAPEUTICS (2018)

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Article Chemistry, Multidisciplinary

High affinity single-chain variable fragments are specific and versatile targeting motifs for extracellular vesicles

Andrea Longatti et al.

NANOSCALE (2018)

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Article Multidisciplinary Sciences

ARMMs as a versatile platform for intracellular delivery of macromolecules

Qiyu Wang et al.

NATURE COMMUNICATIONS (2018)

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Article Multidisciplinary Sciences

Efficient RNA drug delivery using red blood cell extracellular vesicles

Waqas Muhammad Usman et al.

NATURE COMMUNICATIONS (2018)

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Article Medicine, Research & Experimental

VSV-G-Enveloped Vesicles for Traceless Delivery of CRISPR-Cas9

Claudia Montagna et al.

MOLECULAR THERAPY-NUCLEIC ACIDS (2018)

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Article Chemistry, Multidisciplinary

Exosome-Liposome Hybrid Nanoparticles Deliver CRISPR/Cas9 System in MSCs

Yao Lin et al.

ADVANCED SCIENCE (2018)

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Article Chemistry, Multidisciplinary

Recombinant phosphatidylserine-binding nanobodies for targeting of extracellular vesicles to tumor cells: a plug-and-play approach

Sander A. A. Kooijmans et al.

NANOSCALE (2018)

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Article Biotechnology & Applied Microbiology

Targeted cancer therapy using engineered exosome as a natura drug delivery vehicle

Hosna Gomari et al.

ONCOTARGETS AND THERAPY (2018)

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Article Chemistry, Multidisciplinary

Reprogramming Exosomes as Nanoscale Controllers of Cellular Immunity

Qnqin Cheng et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2018)

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Article Biochemical Research Methods

Delivery of an Artificial Transcription Regulator dCas9-VPR by Extracellular Vesicles for Therapeutic Gene Activation

Dusko Lainscek et al.

ACS SYNTHETIC BIOLOGY (2018)

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Review Cell Biology

Glycosylation of extracellular vesicles: current knowledge, tools and clinical perspectives

Charles Williams et al.

JOURNAL OF EXTRACELLULAR VESICLES (2018)

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Article Chemistry, Multidisciplinary

Exosome as a Vehicle for Delivery of Membrane Protein Therapeutics, PH20, for Enhanced Tumor Penetration and Antitumor Efficacy

Yeonsun Hong et al.

ADVANCED FUNCTIONAL MATERIALS (2018)

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Article Nanoscience & Nanotechnology

Pseudotyping exosomes for enhanced protein delivery in mammalian cells

Conary Meyer et al.

INTERNATIONAL JOURNAL OF NANOMEDICINE (2017)

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Article Chemistry, Multidisciplinary

Cancer-derived exosomes as a delivery platform of CRISPR/Cas9 confer cancer cell tropism-dependent targeting

Seung Min Kim et al.

JOURNAL OF CONTROLLED RELEASE (2017)

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Article Biotechnology & Applied Microbiology

Engineered Exosomes as Vehicles for Biologically Active Proteins

Ulrich Sterzenbach et al.

MOLECULAR THERAPY (2017)

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Article Multidisciplinary Sciences

Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer

Sushrut Kamerkar et al.

NATURE (2017)

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Article Chemistry, Multidisciplinary

Peptide/Cas9 nanostructures for ribonucleoprotein cell membrane transport and gene edition

Irene Lostale-Seijo et al.

CHEMICAL SCIENCE (2017)

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Article Multidisciplinary Sciences

Arginine-rich cell-penetrating peptide-modified extracellular vesicles for active macropinocytosis induction and efficient intracellular delivery

Ikuhiko Nakase et al.

SCIENTIFIC REPORTS (2017)

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Review Chemistry, Multidisciplinary

CRISPR/Cas9-Based Genome Editing for Disease Modeling and Therapy: Challenges and Opportunities for Nonviral Delivery

Hong-Xia Wang et al.

CHEMICAL REVIEWS (2017)

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Article Engineering, Biomedical

Augmented liver targeting of exosomes by surface modification with cationized pullulan

Ryo Tamura et al.

ACTA BIOMATERIALIA (2017)

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Review Engineering, Biomedical

Molecular imaging based on metabolic glycoengineering and bioorthogonal click chemistry

Hong Yeol Yoon et al.

BIOMATERIALS (2017)

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Article Cell Biology

Comprehensive toxicity and immunogenicity studies reveal minimal effects in mice following sustained dosing of extracellular vesicles derived from HEK293T cells

Xiaohua Zhu et al.

JOURNAL OF EXTRACELLULAR VESICLES (2017)

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Article Chemistry, Multidisciplinary

Erythrocyte-Platelet Hybrid Membrane Coating for Enhanced Nanoparticle Functionalization

Diana Dehaini et al.

ADVANCED MATERIALS (2017)

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Article Chemistry, Multidisciplinary

Blood Exosomes Endowed with Magnetic and Targeting Properties for Cancer Therapy

Hongzhao Qi et al.

ACS NANO (2016)

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Review Pharmacology & Pharmacy

Extracellular vesicles for drug delivery

Pieter Vader et al.

ADVANCED DRUG DELIVERY REVIEWS (2016)

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Article Engineering, Biomedical

Efficient production and enhanced tumor delivery of engineered extracellular vesicles

Dionysios C. Watson et al.

BIOMATERIALS (2016)

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Article Chemistry, Multidisciplinary

PEGylated and targeted extracellular vesicles display enhanced cell specificity and circulation time

S. A. A. Kooijmans et al.

JOURNAL OF CONTROLLED RELEASE (2016)

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Article Multidisciplinary Sciences

Designed proteins induce the formation of nanocage-containing extracellular vesicles

Jorg Votteler et al.

NATURE (2016)

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Review Multidisciplinary Sciences

Protein misfolding in the endoplasmic reticulum as a conduit to human disease

Miao Wang et al.

NATURE (2016)

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Article Medicine, Research & Experimental

A New Concept of Enhancing Immuno-Chemothera-peutic Effects Against B16F10 Tumor via Systemic Administration by Taking Advantages of the Limitation of EPR Effect

Yuting Yang et al.

THERANOSTICS (2016)

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Article Multidisciplinary Sciences

Engineering hybrid exosomes by membrane fusion with liposomes

Yuko T. Sato et al.

SCIENTIFIC REPORTS (2016)

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Article Cell Biology

Display of GPI-anchored anti-EGFR nanobodies on extracellular vesicles promotes tumour cell targeting

Sander A. A. Kooijmans et al.

JOURNAL OF EXTRACELLULAR VESICLES (2016)

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Article Ethics

Human Germline CRISPR-Cas Modification: Toward a Regulatory Framework

Niklaus H. Evitt et al.

AMERICAN JOURNAL OF BIOETHICS (2015)

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Article Oncology

Grapefruit-Derived Nanovectors Use an Activated Leukocyte Trafficking Pathway to Deliver Therapeutic Agents to Inflammatory Tumor Sites

Qilong Wang et al.

CANCER RESEARCH (2015)

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Article Biochemistry & Molecular Biology

Stabilization of Exosome-targeting Peptides via Engineered Glycosylation

Michelle E. Hung et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2015)

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Article Chemistry, Multidisciplinary

Biodistribution and delivery efficiency of unmodified tumor-derived exosomes

Tyson Smyth et al.

JOURNAL OF CONTROLLED RELEASE (2015)

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Article Chemistry, Multidisciplinary

Active loading into extracellular vesicles significantly improves the cellular uptake and photodynamic effect of porphyrins

Gregor Fuhrmann et al.

JOURNAL OF CONTROLLED RELEASE (2015)

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Article Chemistry, Medicinal

Quantitative Analysis of Tissue Distribution of the B16BL6-Derived Exosomes Using a Streptavidin-Lactadherin Fusion Protein and Iodine-125-Labeled Biotin Derivative After Intravenous Injection in Mice

Masaki Morishita et al.

JOURNAL OF PHARMACEUTICAL SCIENCES (2015)

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Article Multidisciplinary Sciences

Synthesis and applications of RNAs with position-selective labelling and mosaic composition

Yu Liu et al.

NATURE (2015)

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Article Multidisciplinary Sciences

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

Silvana Konermann et al.

NATURE (2015)

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Article Biotechnology & Applied Microbiology

Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

Ayal Hendel et al.

NATURE BIOTECHNOLOGY (2015)

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Review Cell Biology

Ectosomes and exosomes: shedding the confusion between extracellular vesicles

Emanuele Cocucci et al.

TRENDS IN CELL BIOLOGY (2015)

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Review Cell Biology

An Apoptotic 'Eat Me' Signal: Phosphatidylserine Exposure

Katsumori Segawa et al.

TRENDS IN CELL BIOLOGY (2015)

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Article Multidisciplinary Sciences

Combined treatment with a pH-sensitive fusogenic peptide and cationic lipids achieves enhanced cytosolic delivery of exosomes

Ikuhiko Nakase et al.

SCIENTIFIC REPORTS (2015)

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Article Cell Biology

Extracellular vesicle in vivo biodistribution is determined by cell source, route of administration and targeting

Oscar P. B. Wiklander et al.

JOURNAL OF EXTRACELLULAR VESICLES (2015)

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Article Biochemical Research Methods

Maximizing exosome colloidal stability following electroporation

Joshua L. Hood et al.

ANALYTICAL BIOCHEMISTRY (2014)

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Article Biochemistry & Molecular Biology

Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA

Suresh Ramakrishna et al.

GENOME RESEARCH (2014)

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Article Medicine, Research & Experimental

The Absence of CpG in Plasmid DNA-Chitosan Polyplexes Enhances Transfection Efficiencies and Reduces Inflammatory Responses in Murine Lungs

Amaraporn Wongrakpanich et al.

MOLECULAR PHARMACEUTICS (2014)

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Article Chemistry, Multidisciplinary

Electroporation-induced siRNA precipitation obscures the efficiency of siRNA loading into extracellular vesicles

Sander A. A. Kooijmans et al.

JOURNAL OF CONTROLLED RELEASE (2013)

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Article Biotechnology & Applied Microbiology

Systemically Injected Exosomes Targeted to EGFR Deliver Antitumor MicroRNA to Breast Cancer Cells

Shin-ichiro Ohno et al.

MOLECULAR THERAPY (2013)

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Article Oncology

Exosome Targeting of Tumor Antigens Expressed by Cancer Vaccines Can Improve Antigen Immunogenicity and Therapeutic Efficacy

Ryan B. Rountree et al.

CANCER RESEARCH (2011)

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Article Biotechnology & Applied Microbiology

Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes

Lydia Alvarez-Erviti et al.

NATURE BIOTECHNOLOGY (2011)

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Article Biochemistry & Molecular Biology

Direct integration of cell-free-synthesized connexin-43 into liposomes and hemichannel formation

Yuki Moritani et al.

FEBS JOURNAL (2010)

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Article Medicine, Research & Experimental

Vaccination of metastatic melanoma patients with autologous dendritic cell (DC) derived-exosomes:: results of thefirst phase I clinical trial

B Escudier et al.

JOURNAL OF TRANSLATIONAL MEDICINE (2005)

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