4.7 Review

Improving abiotic stress tolerance of forage grasses - prospects of using genome editing

Related references

Note: Only part of the references are listed.
Review Biochemical Research Methods

Getting better all the time - recent progress in the development of CRISPR/Cas-based tools for plant genome engineering

Niklas Capdeville et al.

Summary: Since their first adaptation for plant genome editing, CRISPR-Cas system nucleases and tools have revolutionized the field, enabling precise induction of predefined modifications and the generation of more efficient base editors. Prime editors have also been optimized for plant applications, allowing accurate substitutions, insertions, and deletions. Recent progress in chromosome restructuring allows not only genetic linkages and breakage, but also promoter swapping.

CURRENT OPINION IN BIOTECHNOLOGY (2023)

Editorial Material Plant Sciences

Plant development meets climate emergency - it's time to plant an apple tree

Keiko Sugimoto et al.

CURRENT OPINION IN PLANT BIOLOGY (2022)

Article Biochemistry & Molecular Biology

Functional Characterization of Tomato Phytochrome A and B1B2 Mutants in Response to Heat Stress

Islam M. Y. Abdellatif et al.

Summary: Heat stress is a prevalent negative factor affecting plant growth and development. In this study, the response of tomato phyA and phyB1B2 mutants to high temperatures at different growth stages was investigated. The mutants showed tolerance to heat stress during the seed germination and vegetative growth stages, while their response during the flowering stage was dependent on the duration of heat exposure. The vegetative growth stage demonstrated the highest tolerance, with increased membrane stability and water preservation achieved through stomatal closure regulation. Additionally, both mutants upregulated heat-responsive genes and the phyA mutant showed enhanced proline levels and lower malondialdehyde accumulation.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Review Biochemistry & Molecular Biology

ICE-CBF-COR Signaling Cascade and Its Regulation in Plants Responding to Cold Stress

Delight Hwarari et al.

Summary: Plants have evolved complex mechanisms to adapt to cold stress, regulated by the ICE-CBF-COR signaling pathway. This pathway regulates the expression of transcription factors and proteins, enhancing cold stress tolerance in plants.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Article Biochemistry & Molecular Biology

A well-supported nuclear phylogeny of Poaceae and implications for the evolution of C4 photosynthesis

Weichen Huang et al.

Summary: Poaceae (the grasses) is a economically important angiosperm family, with a wide global distribution and diverse ecosystems. A comprehensive analysis of genomic and transcriptomic data was conducted to reconstruct the phylogeny of Poaceae, providing strong support for the monophyly of 11 subfamilies and suggesting multiple independent origins of C4 photosynthesis. This study offers valuable resources and insights for evolutionary analyses of the grass family.

MOLECULAR PLANT (2022)

News Item Biotechnology & Applied Microbiology

GABA-enriched tomato is first CRISPR-edited food to enter market

Emily Waltz

NATURE BIOTECHNOLOGY (2022)

Editorial Material Plant Sciences

Plant responses and adaptations to a changing climate

John C. Cushman et al.

PLANT JOURNAL (2022)

Review Genetics & Heredity

An Epigenetic Alphabet of Crop Adaptation to Climate Change

Francesco Guarino et al.

Summary: Crop adaptation to climate change is influenced by epigenetic mechanisms, which are still poorly understood in non-model plants. This review summarizes the recent findings on crop epigenetic responses to climate change and presents a speculative viewpoint on the underlying epigenetic mechanisms. Understanding these mechanisms will facilitate the design and cultivation of crop varieties with diverse tolerance/resistance to stresses.

FRONTIERS IN GENETICS (2022)

Article Biotechnology & Applied Microbiology

Genomic Editing: The Evolution in Regulatory Management Accompanying Scientific Progress

Maria Florencia Goberna et al.

Summary: Argentina has implemented a new regulation for genome-editing products, which applies to all organisms and is not linked to commercial GMO regulations. This regulation provides certainty for local researchers and developers, and encourages more innovation and development.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2022)

Review Genetics & Heredity

Gene drives gaining speed

Ethan Bier

Summary: This Review discusses the impact of CRISPR-based gene-drive systems on controlling populations such as insect vectors of disease. It summarizes progress in designing full-drive elements, updating genetic elements, and mitigating strategies, while emphasizing the importance of ethical and social considerations in advancing these technologies towards field implementation.

NATURE REVIEWS GENETICS (2022)

Article Plant Sciences

Developing climate-resilient crops: improving plant tolerance to stress combination

Rosa M. Rivero et al.

Summary: Global warming and climate change are leading to an increase in abiotic stresses impacting plants, leading to decreased crop yields and food shortages. The combination of different stresses on plants caused by climate change necessitates further research to understand and mitigate these effects.

PLANT JOURNAL (2022)

Editorial Material Plant Sciences

Updates on gene editing and its applications

Holger Puchta et al.

PLANT PHYSIOLOGY (2022)

Article Green & Sustainable Science & Technology

Development of Novel Rice Germplasm for Salt-Tolerance at Seedling Stage Using CRISPR-Cas9

Xiaoli Han et al.

Summary: In this study, CRISPR/Cas9 technology was used to edit the main effect gene OsRR22 in rice, resulting in a new germplasm with OsRR22 gene knockout. The mutant lines showed significant differences in plant growth and biomass compared to the wildtype after salt treatment, indicating that the OsRR22 mutation may improve salt tolerance in rice.

SUSTAINABILITY (2022)

Article Agronomy

Relationship between Freezing Tolerance and Leaf Growth during Acclimation in Winter Wheat

Kristina Jaskune et al.

Summary: Winter hardiness in wheat is influenced by various environmental factors, with freezing tolerance being a key factor. In this study, the growth response of different wheat cultivars to low temperatures during the acclimation period was studied. The results showed variations in thermal growth patterns among the cultivars, and a correlation was found between leaf growth parameters and freezing tolerance. The findings highlight the importance of growth rate patterns in determining winter hardiness.

AGRONOMY-BASEL (2022)

Review Plant Sciences

Tissue Culture and Somatic Embryogenesis in Warm-Season Grasses-Current Status and Its Applications: A Review

Melody Ballitoc Muguerza et al.

Summary: Warm-season grasses, as C-4 plants, are highly productive in terms of biomass. They are widely used in various agricultural production systems, such as livestock feeds, bioethanol, and turf. However, improving these grasses through conventional breeding is difficult due to their vegetative propagation or asexual mode of reproduction. Recent studies have shown that plant tissue culture system using somatic embryogenesis can complement conventional breeding methods and allow for micropropagation, somaclonal variation, somatic hybridization, genetic transformation, and genome editing.

PLANTS-BASEL (2022)

Article Plant Sciences

CRISPR/Cas9 Mediated Knockout of the OsbHLH024 Transcription Factor Improves Salt Stress Resistance in Rice (Oryza sativa L.)

Mohammad Shah Alam et al.

Summary: The study used the CRISPR/Cas9 strategy to investigate the role of OsbHLH024 in rice under salt stress. It was found that OsbHLH024 may play a negative regulatory role in salt stress, helping to better understand the molecular basis of rice production improvement under salt stress.

PLANTS-BASEL (2022)

Article Biology

OsOSCA1.1 Mediates Hyperosmolality and Salt Stress Sensing in Oryza sativa

Yang Han et al.

Summary: This study found that OsOSCA1.1 mediates calcium ion increases and stomatal closure in response to hyperosmolality and salt stress in rice. It plays a critical role in seedling survival and gene expression in shoots. Transcriptomic analysis revealed that OsOSCA1.1-regulated genes are enriched in transcription regulation, hormone response, and phosphorylation.

BIOLOGY-BASEL (2022)

Article Biotechnology & Applied Microbiology

Chromosome-scale assembly and annotation of the perennial ryegrass genome

Istvan Nagy et al.

Summary: In this study, a chromosome-scale genome sequence assembly of perennial ryegrass was reported, which will serve as valuable resources for evolutionary and comparative genomics as well as breeding applications.

BMC GENOMICS (2022)

Review Multidisciplinary Sciences

Molecular, cellular, and developmental foundations of grass diversity

Paula McSteen et al.

Summary: Grasses have been cultivated by humans for various purposes for thousands of years. They have adapted and diversified into approximately 12,000 species, with different genes and traits selected in different crop species. Research on the molecular, cellular, and developmental bases of grain yield and dispersal in grasses is ongoing, and with increasing genomic resources, we expect to gain more insights into their ecological and economic success.

SCIENCE (2022)

Article Multidisciplinary Sciences

The unrecognized value of grass

Bianca Lopez et al.

SCIENCE (2022)

Review Plant Sciences

Sensing Mechanisms: Calcium Signaling Mediated Abiotic Stress in Plants

Tongfei Xu et al.

Summary: This review highlights the early events of stress signaling in plants, focusing on the role of calcium ions as a second messenger. It also raises important questions about the integration of multiple stress conditions and subsequent signaling responses that require further research.

FRONTIERS IN PLANT SCIENCE (2022)

Article Plant Sciences

Effect of Different Salts on Nutrients Uptake, Gene Expression, Antioxidant, and Growth Pattern of Selected Rice Genotypes

Muhammad Farooq et al.

Summary: This study investigated the effects of CaCl2, MgCl2, and NaCl on ion uptake, gene expression, seed germination, antioxidants, plant growth, and biomass in different rice genotypes. The results showed that different salts had differential effects on ion uptake and gene expression in different rice genotypes, with NaCl stress significantly reducing agronomic traits. In terms of salt tolerance, Nagdong exhibited better salt tolerance, while Pokkali responded well to NaCl stress.

FRONTIERS IN PLANT SCIENCE (2022)

Article Biochemistry & Molecular Biology

Genome-Wide Identification, Evolution and Expressional Analysis of OSCA Gene Family in Barley (Hordeum vulgare L.)

Kuijun She et al.

Summary: In this study, the OSCA gene family in barley was identified and its role in abiotic stress response was explored. The results showed that these genes play a crucial role in osmotic regulation and exhibit genetic diversity. These findings provide potential candidates for further functional genomic study and contribute to genetically improving stress tolerance in barley and other crops.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Review Biochemistry & Molecular Biology

Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops

Miguel Angel Villalobos-Lopez et al.

Summary: The major challenges faced by agriculture in the twenty-first century are climate change-induced droughts, water scarcity, flooding, poorer soils, and extreme temperatures. However, most crops are not tolerant to extreme climatic conditions. The aim is to breed or engineer crops that can tolerate abiotic stress and provide higher yields in a world with hunger and a growing population.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Article Plant Sciences

Climate change-associated multifactorial stress combination: A present challenge for our ecosystems

Lidia S. Pascual et al.

Summary: Human activities have a negative impact on Earth's ecosystems and biodiversity, leading to global warming, climate change, and man-made pollution. Studies have shown that when different stress factors act simultaneously, there is a dramatic decline in the health of plants and ecosystems. Therefore, it is important to reduce pollutants, develop strategies to address global warming, and enhance the tolerance of plants to multiple stress factors.

JOURNAL OF PLANT PHYSIOLOGY (2022)

Article Plant Sciences

Domestication-associated PHYTOCHROME C is a flowering time repressor and a key factor determining Setaria as a short-day plant

Hailong Wang et al.

Summary: Phytochromes play a crucial role in the domestication of Setaria, and the mutants identified in this study exhibited early heading dates and a loss of response to short-day photoperiod. The results suggest that these mutants have the potential to serve as a model for Setaria and for creating an efficient indoor cultivation system for research.

NEW PHYTOLOGIST (2022)

Review Plant Sciences

A critical review on plant annexin: Structure, function, and mechanism

Xiaoxia Wu et al.

Summary: Plant annexins are a conserved protein family involved in regulating various aspects of plant growth, development, and stress responses. Recent studies have made significant progress in understanding the functional roles of plant annexins, but there are still many interesting questions that need further elucidation.

PLANT PHYSIOLOGY AND BIOCHEMISTRY (2022)

Editorial Material Multidisciplinary Sciences

Toward product-based regulation of crops

Fred Gould et al.

SCIENCE (2022)

Article Plant Sciences

Identification of the genes at S and Z reveals the molecular basis and evolution of grass self-incompatibility

Rowan Herridge et al.

Summary: Self-incompatibility is a characteristic of flowering plants, and grasses have a unique two-locus system to control self-recognition. By analyzing genome data from ryegrass and other grass species, it was found that DUF247 genes and SP/ZP genes are present at the S and Z loci in self-incompatibility species, acting as male and female components. These genes are highly variable, but key secondary structure features are conserved. The time of formation of specificity groups is related to the divergence of grass subfamilies.

FRONTIERS IN PLANT SCIENCE (2022)

Review Genetics & Heredity

Comprehending the evolution of gene editing platforms for crop trait improvement

Priyanka Dhakate et al.

Summary: The CRISPR/Cas system has evolved from a mechanism for bacterial and archaeal adaptive immunity to a powerful tool for genome editing. By combining with next-generation sequencing methods, numerous gene editing-based crop improvement technologies have been developed, revolutionizing plant genome editing methodologies and enabling the improvement of crop traits through genome modification.

FRONTIERS IN GENETICS (2022)

Review Cell Biology

Recent Progress and Future Prospect of CRISPR/Cas-Derived Transcription Activation (CRISPRa) System in Plants

Xiao Ding et al.

Summary: The CRISPR/Cas system has advantages in gene regulation and provides powerful tools for genetic manipulation by activating endogenous genes. The limitations of traditional gene overexpression methods can be overcome by the CRISPRa system to achieve multiple gene activation.

CELLS (2022)

Article Agronomy

Multiplex CRISPR/Cas9-mediated genome editing to address drought tolerance in wheat

Naglaa A. Abdallah et al.

Summary: Genome editing using a multiplex sgRNA-CRISPR/Cas9 system has been utilized to generate wheat mutants with TaSal1 gene mutations, resulting in phenotypic changes such as rolled leaves and bent stems. Anatomical and microscopic analysis of the mutated plants showed alterations in stomata and bulliform cells. This study demonstrates the efficiency of multiplex sgRNA-CRISPR/Cas9 genome editing in inducing mutations in hexaploid wheat.

GM CROPS & FOOD-BIOTECHNOLOGY IN AGRICULTURE AND THE FOOD CHAIN (2022)

Article Agronomy

Farm income and production impacts from the use of genetically modified (GM) crop technology 1996-2020

Graham Brookes

Summary: This paper provides updated estimates for the global value of using genetically modified (GM) crop technology in agriculture. It concludes that the technology has significantly increased farm incomes and global production levels of main crops. The economic benefits have been divided between farmers in developing and developed countries, with yield and production gains contributing the most to the overall income gains.

GM CROPS & FOOD-BIOTECHNOLOGY IN AGRICULTURE AND THE FOOD CHAIN (2022)

Article Food Science & Technology

Criteria for risk assessment of plants produced by targeted mutagenesis, cisgenesis and intragenesis

Ewen Mullins et al.

Summary: EFSA has proposed six main criteria to assist in the risk assessment of plants produced through targeted mutagenesis, cisgenesis, and intragenesis. These criteria focus on the molecular characterization of the genetic modification, including the presence of exogenous DNA sequences, the source of these sequences, the type of integration, and the interruption of endogenous plant genes. Additional criteria are considered for evaluating the history of safe use and the structure/function of the introduced allele.

EFSA JOURNAL (2022)

Article Food Science & Technology

Updated scientific opinion on plants developed through cisgenesis and intragenesis

Ewen Mullins et al.

Summary: With the development of New Genomic Techniques (NGTs), plants developed through cisgenesis and intragenesis can be obtained with precise insertion of desired sequences in the genome. The current guidelines for risk assessment of these plants are partially applicable and sufficient. No new risks are identified in cisgenic and intragenic plants obtained with NGTs compared to conventional breeding and Established Genomic Techniques (EGTs).

EFSA JOURNAL (2022)

Article Biotechnology & Applied Microbiology

Agrobacterium- and a single Cas9-sgRNA transcript system-mediated high efficiency gene editing in perennial ryegrass

Rahul Kumar et al.

Summary: Genome editing technologies have a significant impact on the genetic improvement of perennial ryegrass. By utilizing the maize Ubiquitin 1 (ZmUbi1) promoter to control the expression of Cas9 and sgRNA, bi-allelic mutant plants of perennial ryegrass can be efficiently produced. This approach can also be applied to other related grass species.

FRONTIERS IN GENOME EDITING (2022)

Article Plant Sciences

Biofortified tomatoes provide a new route to vitamin D sufficiency

Jie Li et al.

Summary: Vitamin D insufficiency is a significant public health issue, and this study has successfully engineered tomato lines that accumulate provitamin D-3, providing a new dietary source and the potential for supplement production.

NATURE PLANTS (2022)

Article Agronomy

Genome-wide markers for seed yield and disease resistance in perennial ryegrass

Kristina Jaskune et al.

Summary: This study evaluated seed yield-related traits and resistance to crown rust in perennial ryegrass cultivars and ecotypes, and identified genome-wide markers associated with these traits. The findings revealed variations in seed yield and crown rust resistance in perennial ryegrass, and identified genes with potential roles in disease resistance.

CROP JOURNAL (2022)

Article Plant Sciences

Improvement of base editors and prime editors advances precision genome engineering in plants

Kai Hua et al.

Summary: CRISPR/CRISPR-associated protein-mediated gene editing has revolutionized biomedical research and plant and animal breeding. This review focuses on two recently developed approaches, base editing and prime editing, that efficiently introduce precise edits in plants without the need for double-stranded breaks or donor DNA templates. The article also discusses recent progress in base editing of plant organellar genomes and highlights the impact of these precision genome editing tools on basic plant research and crop breeding.

PLANT PHYSIOLOGY (2022)

Article Plant Sciences

Climate change-associated multifactorial stress combination: A present challenge for our ecosystems

Lidia S. Pascual et al.

Summary: Global warming, climate change, and pollution negatively impact Earth's ecosystems and biodiversity, leading to dramatic declines in plant and ecosystem health under multiple stress conditions.

JOURNAL OF PLANT PHYSIOLOGY (2022)

Article Multidisciplinary Sciences

Factors influencing adoption of integrated soil fertility management technologies by smallholder farmers in Ghana

Moses Kwadzo et al.

Summary: The study found that only a minority of respondents adopted the full set of integrated soil fertility management technologies, and that agroecological zone is a significant factor influencing smallholder farmers' adoption of these technologies.

HELIYON (2021)

Review Biochemistry & Molecular Biology

Plant Dehydrins: Expression, Regulatory Networks, and Protective Roles in Plants Challenged by Abiotic Stress

Zhenping Sun et al.

Summary: Dehydrins, also known as Group II late embryogenesis abundant (LEA) proteins, are highly hydrophilic classic intrinsically disordered proteins that play a protective role in plants exposed to abiotic stress. They are involved in seed maturation, plant stress tolerance, and potentially in stabilizing DNA conformations and protecting membranes and proteins. The regulatory networks of dehydrin gene expression, including ABA, MAP kinase cascade, and Ca2+ signaling pathways, form a complex, diverse system that may regulate the same dehydrin.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Review Biochemistry & Molecular Biology

Genome engineering for crop improvement and future agriculture

Caixia Gao

Summary: Genome editing has the potential to revolutionize plant breeding and address the challenge of feeding a growing population in the face of climate change. Newly developed techniques and strategies offer significant opportunities for plant improvement and crop production, but challenges must be overcome to realize the full potential of this technology.
Article Multidisciplinary Sciences

Enhancement of drought tolerance in rice by silencing of the OsSYT-5 gene

Sudha Shanmugam et al.

Summary: By silencing the OsSYT-5 gene, rice plants showed improved tolerance to water deficit stress, enhanced photosynthetic rate, reduced stomatal conductance, and transpiration. Additionally, the elevated ABA content in the leaves of transgenic rice altered the expression of multiple genes associated with ABA-related stress signaling.

PLOS ONE (2021)

Article Multidisciplinary Sciences

de novo transcriptomic profiling of differentially expressed genes in grass halophyte Urochondra setulosa under high salinity

Anita Mann et al.

Summary: This study investigated the gene expression of Urochondra setulosa leaves at different salt concentrations using transcriptomics. The findings suggest that the plant regulates ion homeostasis and signaling through transcription at high salt concentrations. Furthermore, genes related to salt stress response were identified, providing insights into the mechanisms of plants in coping with extreme saline conditions.

SCIENTIFIC REPORTS (2021)

Article Plant Sciences

The CLV3 Homolog in Setaria viridis Selectively Controls Inflorescence Meristem Size

Chuanmei Zhu et al.

Summary: The CLAVATA pathway regulates meristem size during inflorescence development in plants through peptide ligands encoded by CLE genes. Different plant species may be controlled by different CLE peptides, suggesting diversity in CLE responses among grasses.

FRONTIERS IN PLANT SCIENCE (2021)

Review Agronomy

Induced Polyploidy: A Tool for Forage Species Improvement

Saeed Rauf et al.

Summary: Polyploidy refers to having more than two sets of chromosomes. It can be artificially induced in plants through various methods, leading to increased gene scope and expression, impacting yield and product quality. Experimentally induced polyploid germplasm is a valuable tool for understanding gene expression, but challenges related to genome and epigenome stability need to be addressed.

AGRICULTURE-BASEL (2021)

Article Plant Sciences

Identification of Candidate Genes for Self-Compatibility in Perennial Ryegrass (Lolium perenne L.)

Claudio Cropano et al.

Summary: The study identified a locus causing self-compatibility in perennial ryegrass, mapped to LG 5 through low-resolution and fine mapping, providing diagnostic markers for introgressing self-compatibility into elite germplasm of forage grasses.

FRONTIERS IN PLANT SCIENCE (2021)

Article Plant Sciences

Transcriptome and Metabolome Analyses Reveal Potential Salt Tolerance Mechanisms Contributing to Maintenance of Water Balance by the Halophytic Grass Puccinellia nuttalliana

Maryamsadat Vaziriyeganeh et al.

Summary: The study revealed changes in transcript levels and metabolites in P. nuttalliana under salt stress, suggesting potential mechanisms for salt tolerance. Alterations in membrane lipid composition in roots and the enrichment of the TCA cycle in leaves highlight important strategies for maintaining cellular homeostasis and coping with salt stress. Additionally, the study identified key transcription factors and metabolites that may contribute to water balance maintenance and survival under high salinity conditions.

FRONTIERS IN PLANT SCIENCE (2021)

Review Agronomy

Grass and legume breeding matching the future needs of European grassland farming

Odd Arne Rognli et al.

Summary: Plant breeding is crucial to produce new grassland varieties that can adapt to climate change. Increasing intra- and interspecific diversity and expanding genetic diversity are recommended for different regions to speed up the development of cultivars with proper adaptation to future climate conditions. Novel technologies such as genome-enabled selection and high-throughput phenotyping will play a major role in this context.

GRASS AND FORAGE SCIENCE (2021)

Review Biochemistry & Molecular Biology

Current Understanding of bHLH Transcription Factors in Plant Abiotic Stress Tolerance

Jianrong Guo et al.

Summary: bHLH proteins are a widespread transcription factor class in eukaryotes, regulating plant developmental and metabolic processes by binding to specific positions on their promoters. They play vital roles in plant responses to abiotic stresses, and future studies and applications of bHLH genes in plant breeding are promising.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Article Biochemistry & Molecular Biology

A Fructan Exohydrolase from Maize Degrades Both Inulin and Levan and Co-Exists with 1-Kestotriose in Maize

Silin Wu et al.

Summary: A Zm-6&1-FEH2 enzyme related to cell wall invertase has been identified in maize, showing substrate specificity for inulin and levan and being localized in the apoplast. The enzyme can hydrolyze 1-kestotriose, an oligofructan, and its expression is influenced by plant development, drought, and abscisic acid.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Review Biochemistry & Molecular Biology

Regulation of Plant Responses to Salt Stress

Shuangshuang Zhao et al.

Summary: Plants adapt to high-salt environments by regulating ion balance, activating osmotic stress pathways, mediating plant hormone signaling, and adjusting cytoskeleton dynamics and cell wall composition in response to salt stress signals. Understanding these mechanisms is crucial for improving agricultural crop yields.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Article Biochemistry & Molecular Biology

Simultaneous gene editing of three homoeoalleles in self-incompatible allohexaploid grasses

Liang Zhang et al.

Summary: This study successfully introduced targeted mutations in all three homoeoalleles of two genes in tall fescue using both CRISPR/Cas9 and LbCas12a systems, demonstrating the feasibility of generating loss-of-function mutants in T0 generation polyploid perennial grasses.

JOURNAL OF INTEGRATIVE PLANT BIOLOGY (2021)

Article Multidisciplinary Sciences

A trade-off between plant and soil carbon storage under elevated CO2

C. Terrer et al.

Summary: Analysis of eCO2 experiments shows that the impact on SOC storage is best explained by a negative relationship with plant biomass, leading to a trade-off between different ecosystems. This highlights the need to revise projections of SOC in climate models.

NATURE (2021)

Letter Biotechnology & Applied Microbiology

Establishing in planta haploid inducer line by edited SiMTL in foxtail millet (Setaria italica)

Zixiang Cheng et al.

PLANT BIOTECHNOLOGY JOURNAL (2021)

Review Plant Sciences

Plant heat stress: Concepts directing future research

S. V. Krishna Jagadish et al.

Summary: This article highlights four key areas for improving our understanding of plant heat stress responses: greater integration of results and tools across different approaches, associating plant responses with tissue temperatures, enhancing understanding of how heat stress affects plant reproduction, and integrating heat stress recovery into breeding programs.

PLANT CELL AND ENVIRONMENT (2021)

Article Biochemical Research Methods

Regulatory approaches for genome edited agricultural plants in select countries and jurisdictions around the world

Jon Entine et al.

Summary: Genome editing in agriculture and food has resulted in new and improved crops and products. The commercialization of these may or may not require approval from regulatory authorities depending on the country or region. This paper provides an overview of the regulatory landscape governing genome edited agriculture and food products in selected countries and regions.

TRANSGENIC RESEARCH (2021)

Editorial Material Biotechnology & Applied Microbiology

Biotechnology and Biosafety Policy at OECD: Future Trends

Peter W. E. Kearns et al.

Summary: The OECD Council Recommendation on Recombinant DNA Safety Considerations has been in force since 1986, outlining safety assessment practices for agricultural and environmental biotechnology that countries should have in place. This article suggests potential updates to make it more suitable for the modern era.

TRENDS IN BIOTECHNOLOGY (2021)

Review Agronomy

Implications of the EFSA Scientific Opinion on Site Directed Nucleases 1 and 2 for Risk Assessment of Genome-Edited Plants in the EU

Nils Rostoks

Summary: Genome editing is a technique for making targeted changes in genomes using site-directed nucleases. Unlike transgenesis, it specifically alters endogenous sequences. The regulation of genome-edited organisms in the EU is strict, with practical implications for stakeholders still unclear.

AGRONOMY-BASEL (2021)

Review Plant Sciences

Fructan Structure and Metabolism in Overwintering Plants

Midori Yoshida

Summary: In winter, plants use fructan as an energy source, storing and degrading it in different ways to ensure survival during winter and regeneration in spring. Fructan synthase genes and FEH genes have been isolated in recent years, and FEHs control various fructan metabolism through different physiological responses.

PLANTS-BASEL (2021)

Review Agriculture, Dairy & Animal Science

Review: New feeds and new feeding systems in intensive and semi-intensive forage-fed ruminant livestock systems

J. M. Moorby et al.

Summary: This review article discusses opportunities to improve nutrient capture by ruminant livestock through new feeds and feeding systems, focusing on intensive and semi-intensive systems. It highlights mechanisms for enhancing the performance and efficiencies of individual animals and production systems, addressing aspects such as improving nitrogen use efficiency and reducing enteric methane production. Additionally, it explores the potential for using home-grown crops or novel feedstuffs as alternative sources to reduce reliance on unsustainably grown soybeans.

ANIMAL (2021)

Article Plant Sciences

Overexpression of ZmDHN11 could enhance transgenic yeast and tobacco tolerance to osmotic stress

Huining Ju et al.

Summary: ZmDHN11, a maize group II LEA protein, protects protein activity and confers osmotic stress resistance on transgenic yeast and tobacco.

PLANT CELL REPORTS (2021)

Review Plant Sciences

Heat Shock Signaling in Land Plants: From Plasma Membrane Sensing to the Transcription of Small Heat Shock Proteins

Baptiste Bourgine et al.

Summary: Heat stress events limit crop productivity, leading plants to accumulate heat-shock proteins (HSPs) for thermal protection. HSP chaperones play a critical role in regulating protein folding and preventing misfolded protein formation, thereby avoiding heat-induced cell death. The HSP20 family is tightly repressed at low temperatures, suggesting a costly mechanism that can become detrimental under unnecessary conditions.

FRONTIERS IN PLANT SCIENCE (2021)

Article Agronomy

Identification of no pollen 1 provides a candidate gene for heterosis utilization in foxtail millet (Setaria italica L.)

Wei Zhang et al.

Summary: In this study, a new male-sterility gene SiNP1 was identified in foxtail millet by analyzing a male-sterile mutant sinp1, which encodes a putative glucose-methanol-choline oxidoreductase affecting pollen wall development. Gene editing and metabolome analysis further revealed key metabolic pathways were disrupted in the sinp1 mutant.

CROP JOURNAL (2021)

Letter Biotechnology & Applied Microbiology

European Court of Justice delivers no justice to Europe on genome-edited crops

Alan H. Schulman et al.

PLANT BIOTECHNOLOGY JOURNAL (2020)

Review Plant Sciences

Desiccation Tolerance: Avoiding Cellular Damage During Drying and Rehydration

Melvin J. Oliver et al.

ANNUAL REVIEW OF PLANT BIOLOGY, VOL 71, 2020 (2020)

Article Agriculture, Multidisciplinary

Prospects for F1 hybrid production in ryegrass

Rowan P. Herridge et al.

NEW ZEALAND JOURNAL OF AGRICULTURAL RESEARCH (2020)

Letter Biotechnology & Applied Microbiology

Targeted mutagenesis in ryegrass (Lolium spp.) using the CRISPR/Cas9 system

Yunwei Zhang et al.

PLANT BIOTECHNOLOGY JOURNAL (2020)

Article Multidisciplinary Sciences

Intertwined signatures of desiccation and drought tolerance in grasses

Jeremy Pardo et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

Article Agriculture, Multidisciplinary

Drought stress response of Westerwolths ryegrass (Lolium multiflorum ssp. multiflorum) cultivars differing in their ploidy level

Olakunle Kelvin Akinroluyo et al.

ZEMDIRBYSTE-AGRICULTURE (2020)

Article Plant Sciences

CRISPR-Cas9 mediated genome editing of drought and salt tolerance (OsDST) gene in indica mega rice cultivar MTU1010

V. V. Santosh Kumar et al.

PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS (2020)

Review Biotechnology & Applied Microbiology

Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors

Andrew V. Anzalone et al.

NATURE BIOTECHNOLOGY (2020)

Article Biochemistry & Molecular Biology

Cuticular waxes-A shield of barley mutant in CBP20 (Cap-Binding Protein 20) gene when struggling with drought stress

Agata Daszkowska-Golec et al.

PLANT SCIENCE (2020)

Review Cell Biology

Applications of CRISPR-Cas in agriculture and plant biotechnology

Haocheng Zhu et al.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2020)

Review Plant Sciences

Heat tolerance in vegetables in the current genomic era: an overview

Saba Aleem et al.

PLANT GROWTH REGULATION (2020)

Article Plant Sciences

The Halophyte Seashore Paspalum Uses Adaxial Leaf Papillae for Sodium Sequestration

John J. Spiekerman et al.

PLANT PHYSIOLOGY (2020)

Review Plant Sciences

Drought and Salinity Stress Responses and Microbe-Induced Tolerance in Plants

Ying Ma et al.

FRONTIERS IN PLANT SCIENCE (2020)

Article Plant Sciences

Leaf anatomical responses and chemical composition of warm-season turfgrasses to increasing salinityy

Manuel R. Chavarria et al.

CURRENT PLANT BIOLOGY (2020)

Review Multidisciplinary Sciences

Mechanisms of Plant Responses and Adaptation to Soil Salinity

Chunzhao Zhao et al.

INNOVATION (2020)

Article Plant Sciences

Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type

Fernanda Gabriela Gonzalez et al.

JOURNAL OF EXPERIMENTAL BOTANY (2019)

Article Biochemical Research Methods

Are current EU policies on GMOs justified?

Andreas T. Christiansen et al.

TRANSGENIC RESEARCH (2019)

Article Multidisciplinary Sciences

Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems

Ashot Papikian et al.

NATURE COMMUNICATIONS (2019)

Review Plant Sciences

The Rise of Apomixis in Natural Plant Populations

Diego Hojsgaard et al.

FRONTIERS IN PLANT SCIENCE (2019)

Article Agronomy

Twenty-one years of using insect resistant (GM) maize in Spain and Portugal: farm-level economic and environmental contributions

Graham Brookes

GM CROPS & FOOD-BIOTECHNOLOGY IN AGRICULTURE AND THE FOOD CHAIN (2019)

Review Biotechnology & Applied Microbiology

Breeding crops to feed 10 billion

Lee T. Hickey et al.

NATURE BIOTECHNOLOGY (2019)

Review Biotechnology & Applied Microbiology

Roads Forward for European GMO Policy-Uncertainties in Wake of ECJ Judgment Have to be Mitigated by Regulatory Reform

Martin Wasmer

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2019)

Review Biochemistry & Molecular Biology

The Function of Inositol Phosphatases in Plant Tolerance to Abiotic Stress

Qi Jia et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019)

Review Multidisciplinary Sciences

Genetic strategies for improving crop yields

Julia Bailey-Serres et al.

NATURE (2019)

Article Agriculture, Multidisciplinary

Differences in salt tolerance between diploid and autotetraploid lines of Lolium multiflorum at the germination and vegetative stages

Olakunle Kelvin Akinroluyo et al.

ZEMDIRBYSTE-AGRICULTURE (2019)

Article Biochemistry & Molecular Biology

EGR2 phosphatase regulates OST1 kinase activity and freezing tolerance in Arabidopsis

Yanglin Ding et al.

EMBO JOURNAL (2019)

Article Multidisciplinary Sciences

A male-expressed rice embryogenic trigger redirected for asexual propagation through seeds

Imtiyaz Khanday et al.

NATURE (2019)

Article Environmental Sciences

Greenhouse gas emissions from synthetic nitrogen manufacture and fertilization for main upland crops in China

Rushan Chai et al.

CARBON BALANCE AND MANAGEMENT (2019)

Article Biotechnology & Applied Microbiology

Multiplexed CRISPR/Cas9-mediated metabolic engineering of γ-aminobutyric acid levels in Solanum lycopersicum

Rui Li et al.

PLANT BIOTECHNOLOGY JOURNAL (2018)

Article Biotechnology & Applied Microbiology

Low-gluten, nontransgenic wheat engineered with CRISPR/Cas9

Susana Sanchez-Leon et al.

PLANT BIOTECHNOLOGY JOURNAL (2018)

Article Environmental Sciences

Grasslands may be more reliable carbon sinks than forests in California

Pawlok Dass et al.

ENVIRONMENTAL RESEARCH LETTERS (2018)

Editorial Material Biochemistry & Molecular Biology

A Footprint of Plant Desiccation Tolerance. Does It Exist?

Henk W. M. Hilhorst et al.

MOLECULAR PLANT (2018)

Article Multidisciplinary Sciences

Mutations in a subfamily of abscisic acid receptor genes promote rice growth and productivity

Chunbo Miao et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2018)

Review Plant Sciences

Improving the Yield and Nutritional Quality of Forage Crops

Nicola M. Capstaff et al.

FRONTIERS IN PLANT SCIENCE (2018)

Review Plant Sciences

An Overview of Biomembrane Functions in Plant Responses to High-Temperature Stress

Yue Niu et al.

FRONTIERS IN PLANT SCIENCE (2018)

Letter Biotechnology & Applied Microbiology

EU court casts new plant breeding techniques into regulatory limbo

Kai P. Purnhagen et al.

NATURE BIOTECHNOLOGY (2018)

Letter Biotechnology & Applied Microbiology

A call for science-based review of the European court's decision on gene-edited crops

Fyodor D. Urnov et al.

NATURE BIOTECHNOLOGY (2018)

Review Meteorology & Atmospheric Sciences

Effect of Climate Change on the Yield of Cereal Crops: A Review

Jin Wang et al.

CLIMATE (2018)

Article Plant Sciences

Generation of self-compatible diploid potato by knockout of S-RNase

Mingwang Ye et al.

NATURE PLANTS (2018)

Article Biotechnology & Applied Microbiology

ARGOS8 variants generated by CRISPR-Cas9 improve maize grain yield under field drought stress conditions

Jinrui Shi et al.

PLANT BIOTECHNOLOGY JOURNAL (2017)

Article Plant Sciences

SYTA has positive effects on the heat resistance of Arabidopsis

Qiujie Yan et al.

PLANT GROWTH REGULATION (2017)

Article Plant Sciences

Stomatal and growth responses to hydraulic and chemical changes induced by progressive soil drying

Xiaoqing Li et al.

JOURNAL OF EXPERIMENTAL BOTANY (2017)

Article Biochemistry & Molecular Biology

A Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants

Tomas Cermak et al.

PLANT CELL (2017)

Article Biochemistry & Molecular Biology

Deacclimation may be crucial for winter survival of cereals under warming climate

Marcin Rapacz et al.

PLANT SCIENCE (2017)

Article Multidisciplinary Sciences

CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing

Miguel A. Moreno-Mateos et al.

NATURE COMMUNICATIONS (2017)

Review Plant Sciences

Multifunctional Roles of Plant Dehydrins in Response to Environmental Stresses

Yang Liu et al.

FRONTIERS IN PLANT SCIENCE (2017)

Review Plant Sciences

Halophytes: Potential Resources for Salt Stress Tolerance Genes and Promoters

Avinash Mishra et al.

FRONTIERS IN PLANT SCIENCE (2017)

Article Biochemistry & Molecular Biology

A Gene Encoding a DUF247 Domain Protein Cosegregates with the S Self-Incompatibility Locus in Perennial Ryegrass

Chloe Manzanares et al.

MOLECULAR BIOLOGY AND EVOLUTION (2016)

Review Biotechnology & Applied Microbiology

Genomics of crop wild relatives: expanding the gene pool for crop improvement

Marta Brozynska et al.

PLANT BIOTECHNOLOGY JOURNAL (2016)

Article Biotechnology & Applied Microbiology

Transcriptome profiling of Kentucky bluegrass (Poa pratensis L.) accessions in response to salt stress

B. Shaun Bushman et al.

BMC GENOMICS (2016)

Article Biotechnology & Applied Microbiology

De novo transcriptome sequencing and gene expression profiling of Elymus nutans under cold stress

Juanjuan Fu et al.

BMC GENOMICS (2016)

Review Plant Sciences

Freezing tolerance revisited - effects of variable temperatures on gene regulation in temperate grasses and legumes

Mallikarjuna Rao Kovi et al.

CURRENT OPINION IN PLANT BIOLOGY (2016)

Article Multidisciplinary Sciences

Sensing and signaling of oxidative stress in chloroplasts by inactivation of the SAL1 phosphoadenosine phosphatase

Kai Xun Chan et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2016)

Editorial Material Plant Sciences

Opening the Gates: Insights into Cyclic Nucleotide-Gated Channel-Mediated Signaling

Thomas A. DeFalco et al.

TRENDS IN PLANT SCIENCE (2016)

Review Plant Sciences

Lessons from Domestication: Targeting Cis-Regulatory Elements for Crop Improvement

Gwen Swinnen et al.

TRENDS IN PLANT SCIENCE (2016)

Review Plant Sciences

Abscisic Acid and Abiotic Stress Tolerance in Crop Plants

Saroj K. Sah et al.

FRONTIERS IN PLANT SCIENCE (2016)

Article Plant Sciences

Differential Activation of the Wheat SnRK2 Family by Abiotic Stresses

Hongying Zhang et al.

FRONTIERS IN PLANT SCIENCE (2016)

Article Biochemistry & Molecular Biology

COLD1 Confers Chilling Tolerance in Rice

Yun Ma et al.

Article Biochemistry & Molecular Biology

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

Bernd Zetsche et al.

Review Biochemistry & Molecular Biology

Salt stress sensing and early signalling events in plant roots: Current knowledge and hypothesis

Sergey Shabala et al.

PLANT SCIENCE (2015)

Review Biochemistry & Molecular Biology

The evolution of drought escape and avoidance in natural herbaceous populations

Nicholas J. Kooyers

PLANT SCIENCE (2015)

Article Multidisciplinary Sciences

The fingerprint of climate trends on European crop yields

Frances C. Moore et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)

Article Plant Sciences

Fructan metabolism and changes in fructan composition during cold acclimation in perennial ryegrass

Shamila W. Abeynayake et al.

FRONTIERS IN PLANT SCIENCE (2015)

Article Biochemistry & Molecular Biology

A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging

Marvin E. Tanenbaum et al.

Review Plant Sciences

Interaction of Temperature and Light in the Development of Freezing Tolerance in Plants

Tibor Janda et al.

JOURNAL OF PLANT GROWTH REGULATION (2014)

Article Biochemical Research Methods

Analysis of Differential Expression Patterns of mRNA and Protein During Cold-acclimation and De-acclimation in Arabidopsis

Kentaro Nakaminami et al.

MOLECULAR & CELLULAR PROTEOMICS (2014)

Article Environmental Sciences

Economics of salt-induced land degradation and restoration

M. Qadir et al.

NATURAL RESOURCES FORUM (2014)

Article Biotechnology & Applied Microbiology

Comparative analysis of maize (Zea mays) crop performance: natural variation, incremental improvements and economic impacts

Mark Leibman et al.

PLANT BIOTECHNOLOGY JOURNAL (2014)

Review Biochemistry & Molecular Biology

Overwintering of herbaceous plants in a changing climate. Still more questions than answers

Marcin Rapacz et al.

PLANT SCIENCE (2014)

Article Multidisciplinary Sciences

Expression of a Truncated ATHB17 Protein in Maize Increases Ear Weight at Silking

Elena A. Rice et al.

PLOS ONE (2014)

Article Plant Sciences

The role of seasonal flowering responses in adaptation of grasses to temperate climates

Siri Fjellheim et al.

FRONTIERS IN PLANT SCIENCE (2014)

Letter Cell Biology

Efficient genome editing in plants using a CRISPR/Cas system

Zhengyan Feng et al.

CELL RESEARCH (2013)

Review Biochemistry & Molecular Biology

Physiological, Biochemical, and Molecular Mechanisms of Heat Stress Tolerance in Plants

Mirza Hasanuzzaman et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2013)

Article Plant Sciences

Cloning and characterization of TaSnRK2.3, a novel SnRK2 gene in common wheat

Shanjun Tian et al.

JOURNAL OF EXPERIMENTAL BOTANY (2013)

Letter Biotechnology & Applied Microbiology

Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

Vladimir Nekrasov et al.

NATURE BIOTECHNOLOGY (2013)

Letter Biotechnology & Applied Microbiology

Targeted genome modification of crop plants using a CRISPR-Cas system

Qiwei Shan et al.

NATURE BIOTECHNOLOGY (2013)

Article Biochemistry & Molecular Biology

Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice

Wenzhi Jiang et al.

NUCLEIC ACIDS RESEARCH (2013)

Article Plant Sciences

ABA signal transduction at the crossroad of biotic and abiotic stress responses

Sung Chul Lee et al.

PLANT CELL AND ENVIRONMENT (2012)

Article Multidisciplinary Sciences

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

Giedrius Gasiunas et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2012)

Article Multidisciplinary Sciences

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

Martin Jinek et al.

SCIENCE (2012)

Review Plant Sciences

Gene Expression Profiling of Plants under Salt Stress

A. Jamil et al.

CRITICAL REVIEWS IN PLANT SCIENCES (2011)

Article Genetics & Heredity

BRIEF REPORTS

Arne Rousi Cytological observations Group et al.

HEREDITAS (2010)

Review Genetics & Heredity

Genome editing with engineered zinc finger nucleases

Fyodor D. Urnov et al.

NATURE REVIEWS GENETICS (2010)

Review Plant Sciences

Drought, ozone, ABA and ethylene: new insights from cell to plant to community

Sally Wilkinson et al.

PLANT CELL AND ENVIRONMENT (2010)

Article Multidisciplinary Sciences

Precise genome modification in the crop species Zea mays using zinc-finger nucleases

Vipula K. Shukla et al.

NATURE (2009)

Review Biochemistry & Molecular Biology

Regulatory genes involved in the determination of frost tolerance in temperate cereals

Gabor Galiba et al.

PLANT SCIENCE (2009)

Review Plant Sciences

Plant fructans in stress environments: emerging concepts and future prospects

Ravi Valluru et al.

JOURNAL OF EXPERIMENTAL BOTANY (2008)

Review Plant Sciences

Salinity tolerance in halophytes

Timothy J. Flowers et al.

NEW PHYTOLOGIST (2008)

Review Plant Sciences

Cold stress regulation of gene expression in plants

Viswanathan Chinnusamy et al.

TRENDS IN PLANT SCIENCE (2007)

Article Plant Sciences

Conservation of the salt overly sensitive pathway in rice

Juliana Martinez-Atienza et al.

PLANT PHYSIOLOGY (2007)

Review Plant Sciences

Invited review: Recent advances in genetic transformation of forage and turf grasses

ZY Wang et al.

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT (2006)

Review Biotechnology & Applied Microbiology

Molecular genetics of fructan metabolism in perennial ryegrass

J Chalmers et al.

PLANT BIOTECHNOLOGY JOURNAL (2005)

Review Plant Sciences

Na+ tolerance and Na+ transport in higher plants

M Tester et al.

ANNALS OF BOTANY (2003)