Identification of photosynthetic parameters for superior yield of two super hybrid rice varieties: A cross-scale study from leaf to canopy

Enhancing photosynthetic capacity is widely accepted as critical to advancing crop yield. Therefore, identifying photosynthetic parameters positively related to biomass accumulation in elite cultivars is the major focus of current rice research. In this work, we assessed leaf photosynthetic performance, canopy photosynthesis, and yield attributes of super hybrid rice cultivars Y-liangyou 3218 (YLY3218) and Y-liangyou 5867 (YLY5867) at tillering stage and flowering stage, using inbred super rice cultivars Zhendao11(ZD11) and Nanjing 9108 (NJ9108) as control. A diurnal canopy photosynthesis model was applied to estimate the influence of key environmental factors, canopy attributes, and canopy nitrogen status on daily aboveground biomass increment (AM(DAY)). Results showed that primarily the light-saturated photosynthetic rate at tillering stage contributed to the advancing yield and biomass of super hybrid rice in comparison to inbred super rice, and the light-saturated photosynthetic rate between them was similar at flowering stage. At tillering stage, the higher CO2 diffusion capacity, together with higher biochemical capacity (i.e., maximum carboxylation rate of Rubisco, maximum electron transport rate (J(max)), and triose phosphate utilization rate) favored leaf photosynthesis of super hybrid rice. Similarly, AM(DAY) in super hybrid rice was higher than inbred super rice at tillering stage, and comparable at flowering stage partially due to increased canopy nitrogen concentration (SLNave) of inbred super rice. At tillering stage, model simulation revealed that replacement of J(max) and g(m) in inbred super rice by super hybrid rice always had a positive effect on AM(DAY), and the averaged AM(DAY) increment was 5.7% and 3.4%, respectively. Simultaneously, the 20% enhancement of total canopy nitrogen concentration through the improvement of SLNave (TNC-SLNave) resulted in the highest AM(DAY) across cultivars, with an average increase of 11.2%. In conclusion, the advancing yield performance of YLY3218 and YLY5867 was due to the higher J(max) and g(m) at tillering stage, and TCN-SLNave is a promising target for future super rice breeding programs.

Automated summary

Enhancing the light-saturated photosynthetic rate is critical to advancing crop yield, especially in super hybrid rice. The higher CO2 diffusion capacity, as well as the higher biochemical capacity, favored leaf photosynthesis of super hybrid rice. Additionally, the total canopy nitrogen concentration at the tillering stage also played an important role in determining the photosynthetic rate and yield.