Journal
FRONTIERS IN MICROBIOLOGY
Volume 14, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2023.1162113
Keywords
brown planthopper; fungal pathogen; Aspergillus fumigatus; transcriptomic sequencing; biocontrol
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In this study, a highly pathogenic native fungal strain against brown planthopper (BPH) was isolated and identified as a strain of Aspergillus fumigatus. Transcriptomic sequencing analysis revealed differentially expressed genes involved in host immune defense and cell detoxification in BPH against fungal attack. Knockdown of an upregulated gene encoding a serine protease (NlSPN) significantly decreased BPH survival. Combination of dsRNA injection and fungal infection showed an additive effect on BPH mortality, providing clues for new pest management strategies against BPH.
The brown planthopper (BPH), Nilaparvata lugens, is one of the most destructive pests of rice. Given the threats posed by insecticide resistance to its control, eco-friendly strategies based on microbial pathogens emerged as a promising biocontrol alternative. In the present study, we isolated a native fungal pathogen against BPH from infected BPH cadavers and preliminarily identified as a strain of Aspergillus fumigatus based on morphological and molecular methods. Laboratory bioassay revealed that this fungal strain was highly virulent to BPH both at nymphal and adult stages, with the median lethal times (LT50) of 7.5 and 5.8 days under high conidial concentration of 1 x 10(9) conidia mL(-1). A genome-wide view of gene expressions in BPH against fungal attack was analyzed by transcriptomic sequencing and consequently a large number of differentially expressed genes that mainly involved in host immune defense and cell detoxification were found. RNAi-mediated knockdown of an upregulated gene encoding a serine protease (NlSPN) could cause a significant decrease in BPH survival. Combination of dsRNA injection and fungal infection showed an additive effect on BPH mortality, which provided clues to develop new pest management strategies against BPH.
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