Size-fractionated N2 fixation off the Changjiang Estuary during summer

Recent evidence has shown active N-2 fixation in coastal eutrophic waters, yet the rate and controlling factors remain poorly understood, particularly in large estuaries. The Changjiang Estuary (CE) and adjacent shelf are characterized by fresh, nitrogen-replete Changjiang Diluted Water (CDW) and saline, nitrogen-depletion intruded Kuroshio water (Taiwan Warm Current and nearshore Kuroshio Branch Current), where N-2 fixation may be contributed by different groups (i.e., Trichodesmium and heterotrophic diazotrophs). Here, for the first time, we provide direct measurement of size-fractionated N-2 fixation rates (NFRs) off the CE during summer 2014 using the N-15(2) bubble tracer method. The results demonstrated considerable spatial variations (southern > northern; offshore > inshore) in surface and depth-integrated NFRs, averaging 0.83 nmol N L-1 d(-1) and 24.3 mu mol N m(-2) d(-1), respectively. The highest bulk NFR (99.9 mu mol N m(-2) d(-1); mostly contributed by >10 mu m fraction) occurred in the southeastern East China Sea, where suffered from strong intrusion of the Kuroshio water characterized by low N/P ratio (<10) and abundant Trichodesmium (up to 10.23 x 10(6) trichomes m(-2)). However, low NFR (mostly contributed by <10 mu m fraction) was detected in the CE controlled by the CDW, where NOx concentration (up to 80 mu mol L-1) and N/P ratio (>100) were high and Trichodesmium abundance was low. The >10 mu m fraction accounted for 60% of depth-integrated bulk NFR over the CE and adjacent shelf. We speculated that the present NFR of >10 mu m fraction was mostly supported by Trichodesmium. Spearman rank correlation indicated that the NFR was significantly positively correlated with Trichodesmium abundance, salinity, temperature and Secchi depth, but was negatively with turbidity, N/P ratio, NOx, and chlorophyll a concentration. Our study suggests that distribution and size structure of N-2 fixation off the CE are largely regulated by water mass (intruded Kuroshio water and CDW) movement and associated diazotrophs (particularly Trichodesmium) and nutrient conditions.

Automated summary

Recent evidence has revealed active N-2 fixation in coastal eutrophic waters, particularly in large estuaries like the Changjiang Estuary (CE). The size-fractionated N-2 fixation rates (NFRs) were directly measured off the CE during summer 2014, showing significant spatial variations and different contributors (Trichodesmium and heterotrophic diazotrophs). The distribution and size structure of N-2 fixation were found to be regulated by water mass movement and associated diazotrophs, as well as nutrient conditions.