Journal
FRONTIERS IN MICROBIOLOGY
Volume 14, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2023.1142582
Keywords
Paenibacillus antarcticus; genome; bioemulsifier; levan; Antarctica
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Paenibacillus antarcticus IPAC21, isolated from King George Island in Antarctica, is capable of forming endospores and producing bioemulsifiers. The genome of IPAC21 was sequenced, revealing the presence of genes related to bioemulsifier production, exopolysaccharide biosynthesis, and other metabolic pathways. The bioemulsifier produced by IPAC21 exhibited stability at different NaCl concentrations, low temperatures, and pH values, suggesting its potential application in the petroleum industry.
Paenibacillus antarcticus IPAC21, an endospore-forming and bioemulsifier-producing strain, was isolated from King George Island, Antarctica. As psychrotolerant/psychrophilic bacteria can be considered promising sources for novel products such as bioactive compounds and other industrially relevant substances/compounds, the IPAC21 genome was sequenced using Illumina Hi-seq, and a search for genes related to the production of bioemulsifiers and other metabolic pathways was performed. The IPAC21 strain has a genome of 5,505,124 bp and a G + C content of 40.5%. Genes related to the biosynthesis of exopolysaccharides, such as the gene that encodes the extracellular enzyme levansucrase responsible for the synthesis of levan, the 2,3-butanediol pathway, PTS sugar transporters, cold-shock proteins, and chaperones were found in its genome. IPAC21 cell-free supernatants obtained after cell growth in trypticase soy broth at different temperatures were evaluated for bioemulsifier production by the emulsification index (EI) using hexadecane, kerosene and diesel. EI values higher than 50% were obtained using the three oil derivatives when IPAC21 was grown at 28 degrees C. The bioemulsifier produced by P. antarcticus IPAC21 was stable at different NaCl concentrations, low temperatures and pH values, suggesting its potential use in lower and moderate temperature processes in the petroleum industry.
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