Journal
LAB ON A CHIP
Volume 16, Issue 3, Pages 489-496Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5lc01117e
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Funding
- K-INBRE Developmental Research Project Award from NIH/NIGMS [P20GM103418]
- Innovative Research Award from the Terry C. Johnson Cancer Research Center
- KU
- COBRE Research Project Award [P20GM103638]
- NIH/NCI grant [R21CA186846]
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Tumor-derived circulating exosomes, enriched with a group of tumor antigens, have been recognized as a promising biomarker source for cancer diagnosis via a less invasive procedure. Quantitatively pinpointing exosome tumor markers is appealing, yet challenging. In this study, we developed a simple microfluidic approach (ExoSearch) which provides enriched preparation of blood plasma exosomes for in situ, multiplexed detection using immunomagnetic beads. The ExoSearch chip offers a robust, continuous-flow design for quantitative isolation and release of blood plasma exosomes in a wide range of preparation volumes (10 mu L to 10 mL). We employed the ExoSearch chip for blood-based diagnosis of ovarian cancer by multiplexed measurement of three exosomal tumor markers (CA-125, EpCAM, CD24) using a training set of ovarian cancer patient plasma, which showed significant diagnostic power (a. u. c. = 1.0, p = 0.001) and was comparable with the standard Bradford assay. This work provides an essentially needed platform for utilization of exosomes in clinical cancer diagnosis, as well as fundamental exosome research.
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