Journal
LAB ON A CHIP
Volume 16, Issue 22, Pages 4341-4349Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6lc01078d
Keywords
-
Categories
Funding
- Penn State CTSI Grant from the National Center for Advancing Translational Sciences, National Institutes of Health [UL Tr000127]
- Penn State Engineering for Innovation & Entrepreneurship (ENGINE) Grant
Ask authors/readers for more resources
In response to the urgent need of a field-deployable and highly sensitive malaria diagnosis, we developed a standalone, sample-in-answer-out molecular diagnostic system (AnyMDx) to enable quantitative molecular analysis of blood-borne malaria in low resource areas. The system consists of a durable battery-powered analyzer and a disposable microfluidic compact disc loaded with reagents ready for use. A low power thermal module and a novel fluorescence-sensing module are integrated into the analyzer for real-time monitoring of loop-mediated isothermal nucleic acid amplification ( LAMP) of target parasite DNA. With 10 mu L of raw blood sample, the AnyMDx system automates the nucleic acid sample preparation and subsequent LAMP and real-time detection. Under laboratory conditions with whole-blood samples spiked with cultured Plasmodium falciparum, we achieved a detection limit of similar to 0.6 parasite per mu L, much lower than those for the conventional microscopy and rapid diagnostic tests (similar to 50-100 parasites per mu L). The turnaround time from sample to answer is less than 40 minutes. The AnyMDx is user-friendly requiring minimal technological training. The analyzer and the disposable reagent compact discs are cost-effective, making AnyMDx a potential tool for malaria molecular diagnosis under field settings for malaria elimination.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available