Journal
LAB ON A CHIP
Volume 16, Issue 15, Pages 2955-2962Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6lc00561f
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Funding
- NIH/NIDA [1R01DA035868-01]
- NIH/NCI [1R01CA180083-01]
- Emily Herman Research Fund
- Golfers Against Cancer Foundation
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Here we developed an integrated volumetric bar-chart chip (IV-Chip) technology by integration of our previous V-Chip with a fluid handling design to generate an instrument-free POC device and greatly reduce the detection time and effort. The IV-Chip test requires only 1 mu L of serum separated from finger-prick blood. The serum sample and ELISA reagents are directly loaded into the device using a pipette, and a shift of the two layers of the device generates homogeneous liquid segments in the microfluidic channel. Under vacuum pressure generated by a regular syringe, the segments flow into the ELISA wells in sequence and a sandwich ELISA reaction takes place. As a result of the automated washing and reacting strategy, the IV-Chip allows rapid tests for myocardial infarction biomarkers, and turnaround time is greatly reduced to 15 min. The specificity and accuracy of quantitative multiplex detection of MI biomarkers CK-MB, troponin I and myoglobin, are 87.5% and 95.8%, respectively.
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