Study on Genomics of the Bisphenol A-Degrading Bacterium Pseudomonas sp. P1

As a widespread pollutant, bisphenol A (BPA) has created a serious threat to ecosystem and human health. Therefore, expanding the available microbial resources used to screen highly efficient BPA-degrading bacteria with BPA as the sole carbon source is very important for the removal of this pollutant from the environment. In this study, the BPA degradation rate of Pseudomonas sp. P1 to 30 mg/L was 96.89% within 120 h. Whole genome sequencing showed that the genome of strain P1 was composed of a single circular chromosome with a full length of 6.17 Mb, which contained 5636 predicted coding genes. Comparative genomic analysis showed that strain P1 contained 210 functional genes related to BPA degradation. It was confirmed that BPA degradation genes ferredoxin (bisdA), P450 (bisdB), CotA and Lac in strain P1 were highly expressed under the induction of BPA. Combined with the identification of metabolites, the route of BPA degradation by Pseudomonas was proposed. A new metabolite, 4-vinylphenol, was detected for the first time in pathway I. In pathway II, BPA is directly oxidized to phenol and 4-isopropenyl phenol in the presence of laccase, which is rarely reported in the process of bacterial degradation of BPA. This study confirmed that strain P1 had good tolerance to various environmental factors at the gene level and enriched the degradation mechanism of BPA.

Automated summary

As a widespread pollutant, BPA poses a serious threat to the ecosystem and human health. This study discovered that Pseudomonas sp. P1 has a high BPA degradation rate, with 96.89% degradation within 120 hours. Whole genome sequencing revealed that strain P1 has a single circular chromosome with 6.17 Mb length and 5636 predicted coding genes. Comparative genomic analysis showed that strain P1 contains 210 functional genes related to BPA degradation. Furthermore, the study identified the degradation pathways of BPA by Pseudomonas and discovered a new metabolite, 4-vinylphenol.