Prussian Blue Sensor for Bacteria Detection in Personal Protection Clothing

Biological hazards can be defined as substances that endanger the life of any living organism, most notably humans, and are often referred to as biohazards. Along with the use of personal protective equipment (PPE), early detection of contact is essential for the correct management and resolution of a biological threat, as well as lower mortality rates of those exposed. Herein, Prussian blue (PB) was evaluated as a functional compound applied on polyester knits to act as an on-site sensor for bacteria detection. In order to study the best compound concentration for the intended application, polymeric solutions of 0.5, 1 and 2 g/L were developed. The three conditions tested displayed high abrasion resistance (>2000 cycles). The bacterial sensing capacity of the coated knits was assessed in liquid and solid medium, with the functionalised substrates exhibiting the capability of detecting both Gram-positive and Gram-negative bacteria and changing colours from blue to white. Evaluation of water repellence and chemical penetration resistance and repellence was also performed in polyester functionalised with PB 0.5 and 1 g/L. Both knits showed a hydrophobic behaviour and a capacity to resist to penetration of chemicals and level 3 repellence effect for both acid and base chemicals.

Automated summary

Biological hazards, also known as biohazards, are substances that pose a threat to the life of any organism, especially humans. Early detection of contact and the use of personal protective equipment (PPE) are crucial for managing and reducing mortality rates of biological threats. This study evaluated the use of Prussian blue (PB) as a functional compound on polyester knits to detect bacteria on-site. Different concentrations of PB were tested, and the coated knits showed high abrasion resistance and the ability to detect both Gram-positive and Gram-negative bacteria.