Journal
CHEMBIOCHEM
Volume 16, Issue 5, Pages 752-755Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201500013
Keywords
5-hydroxymethylcytosine; 5-methylcytosine; isotope tracing; LC-MS; MS; RNA modifications
Funding
- Cambridge PhD Training Programme in Chemical Biology and Molecular Medicine
- Wellcome Trust [099232/Z/12/Z]
- Cancer Research UK [11832] Funding Source: researchfish
- Wellcome Trust [104640/Z/14/Z] Funding Source: researchfish
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RNA methylation is emerging as a regulatory RNA modification that could have important roles in the control and coordination of gene transcription and protein translation. Herein, we describe an in vivo isotope-tracing methodology to demonstrate that the ribonucleoside 5-methylcytidine (m(5)C) is subject to oxidative processing in mammals, forming 5-hydroxymethylcytidine (hm(5)C) and 5-formylcytidine (f(5)C). Furthermore, we have identified hm(5)C in total RNA from all three domains of life and in polyA-enriched RNA fractions from mammalian cells. This suggests m(5)C oxidation is a conserved process that could have critical regulatory functions inside cells.
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