Journal
CHEMBIOCHEM
Volume 16, Issue 17, Pages 2420-2431Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201500340
Keywords
cell-free protein synthesis; eukaryotic and prokaryotic lysates; membrane proteins; post-translational modifications; vesicles
Funding
- German Ministry of Education and Research (BMBF, KMU-Innovativ: Biotechnologie-BioChance) [031A511]
Ask authors/readers for more resources
From its start as a small-scale in vitro system to study fundamental translation processes, cell-free protein synthesis quickly rose to become a potent platform for the high-yield production of proteins. In contrast to classical in vivo protein expression, cell-free systems do not need time-consuming cloning steps, and the open nature provides easy manipulation of reaction conditions as well as high-throughput potential. Especially for the synthesis of difficult to express proteins, such as toxic and transmembrane proteins, cell-free systems are of enormous interest. The modification of the genetic code to incorporate non-canonical amino acids into the target protein in particular provides enormous potential in biotechnology and pharmaceutical research and is in the focus of many cell-free projects. Many sophisticated cell-free systems for manifold applications have been established. This review describes the recent advances in cell-free protein synthesis and details the expanding applications in this field.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available