Journal
CELL REPORTS
Volume 42, Issue 6, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2023.112551
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To kill bacteria, bacteriophages (phages) rely on binding to specific receptors and releasing their DNA into the bacterium. Previous beliefs that the bacterial capsule shields cells from phage attack are challenged by a genetic screen. The study shows that the capsule is actually a primary receptor for phage predation, and a secondary receptor-binding event is required for productive infection.
To kill bacteria, bacteriophages (phages) must first bind to a receptor, triggering the release of the phage DNA into the bacterial cell. Many bacteria secrete polysaccharides that had been thought to shield bacterial cells from phage attack. We use a comprehensive genetic screen to distinguish that the capsule is not a shield but is instead a primary receptor enabling phage predation. Screening of a transposon library to select phage-resistant Klebsiella shows that the first receptor-binding event docks to saccharide epitopes in the capsule. We discover a second step of receptor binding, dictated by specific epitopes in an outer membrane protein. This additional and necessary event precedes phage DNA release to establish a productive infection. That such discrete epitopes dictate two essential binding events for phages has profound implications for under-standing the evolution of phage resistance and what dictates host range, two issues critically important to translating knowledge of phage biology into phage therapies.
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