The development of nasal polyps involves early middle meatus mucous remodeling via TGF-01 mediated PAI-1 reduction

Objective: Our study aimed to elucidate the effect of PAI-1 (Plasminogen Activator Inhibitor-1) and t-PA (Tissue-type Plasminogen Activator) in tissue remodeling in nasal polyps patients. Methods: Samples were streamed as early Nasal Polyps (eNP, n = 10) and inferior tissue from the same patient, mature Nasal Polyps (mNP, n = 14), and Control group (n = 15), respectively. Immunohistochemistry and immunofluorescence were applied to detect localization. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and Western blot were used to measure different levels among three groups. The mNP tissue was cultured in vitro and treated with TGF-(31 (Transforming Growth Factor-beta 1) activator, TGF-(31 inhibitor (SB431542), and PAI-1 inhibitor (TM5275); then Western blot, qRT-PCR, and ELISA were used to assess changes. Results: The immunohistochemistry and immunofluorescence showed that PAI-1 expression decreased in eNP and mNP, mainly in epithelium and glands. The transcriptional expression and protein level of TGF-(31/t-PA/PAI-1/Collagen1 were lower in eNP than IT while mNP group demonstrated lower mRNA expression and protein level of TGF-(31/t-PA/PAI-1/Collagen1 than Control group. In mNP tissue culture in vitro, TGF-(31 activator elevated t-PA, PAI-1, and Collagen1 with higher release of PAI-1 and Collagen1 in supernatant, whereas SB431542 suppressed above reactions; TM5275 lowered transcriptional and protein level of Collagen1 in supernatant.

Automated summary

The effect of PAI-1 and t-PA in tissue remodeling in nasal polyps patients was investigated. PAI-1 expression was found to decrease in nasal polyps, particularly in the epithelium and glands. In vitro culture of nasal polyps tissue showed that TGF-(31 activator increased the expression of t-PA, PAI-1, and Collagen1, while SB431542 suppressed these reactions; TM5275 reduced the transcriptional and protein levels of Collagen1 in the supernatant.