4.7 Article

Engineered Biosynthesis of Complex Disorazol Polyketides in a Streamlined Burkholderia thailandensis

Related references

Note: Only part of the references are listed.
Article Microbiology

Engineering of Burkholderia thailandensis strain E264 serves as a chassis for expression of complex specialized metabolites

Zong-Jie Wang et al.

Summary: In this study, a heterologous expression system based on Burkholderia thailandensis E264 strain was constructed to successfully express the biosynthetic gene clusters of disorazol from Sorangium cellulosum So ce12 and rhizoxin from Burkholderia rhizoxinica. The yield of disorazol F-2 was improved by promoter substitution and insertion. This system provides a new option for exploring natural products from different phylogenetic taxa.

FRONTIERS IN MICROBIOLOGY (2022)

Article Biochemistry & Molecular Biology

antiSMASH 6.0: improving cluster detection and comparison capabilities

Kai Blin et al.

Summary: antiSMASH, a tool for detecting biosynthetic gene clusters in microorganisms, has been updated to version 6, which increases the supported cluster types, displays the structure of multi-modular BGCs, adds a new comparison algorithm, allows integration of results from other prediction tools, and improves detection of tailoring enzymes in RiPP clusters.

NUCLEIC ACIDS RESEARCH (2021)

Article Chemistry, Organic

Total Synthesis of (-)-Disorazole C1

Luca Lizzadro et al.

Summary: A scalable and robust synthesis of (-)-disorazole C-1 with high stereoselectivity is described, featuring simple reaction conditions that can produce large quantities of this biologically active compound.

ORGANIC LETTERS (2021)

Article Multidisciplinary Sciences

An in vitro platform for engineering and harnessing modular polyketide synthases

Takeshi Miyazawa et al.

NATURE COMMUNICATIONS (2020)

Article Chemistry, Multidisciplinary

Streamlined Symmetrical Total Synthesis of Disorazole B1 and Design, Synthesis, and Biological Investigation of Disorazole Analogues

K. C. Nicolaou et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2020)

Article Biochemistry & Molecular Biology

RedEx: a method for seamless DNA insertion and deletion in large multimodular polyketide synthase gene clusters

Chaoyi Song et al.

NUCLEIC ACIDS RESEARCH (2020)

Review Chemistry, Multidisciplinary

Evolution and Diversity of Assembly-Line Polyketide Synthases

Aleksandra Nivina et al.

CHEMICAL REVIEWS (2019)

Article Biochemistry & Molecular Biology

The modules of trans-acyltransferase assembly lines redefined with a central acyl carrier protein

Drew A. Vander Wood et al.

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS (2018)

Article Multidisciplinary Sciences

A fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin

Gregory Menchon et al.

NATURE COMMUNICATIONS (2018)

Article Chemistry, Multidisciplinary

Total Syntheses of Disorazoles A1 and B1 and Full Structural Elucidation of Disorazole B1

K. C. Nicolaou et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2017)

Article Multidisciplinary Sciences

Diversity oriented biosynthesis via accelerated evolution of modular gene clusters

Aleksandra Wlodek et al.

NATURE COMMUNICATIONS (2017)

Article Chemistry, Multidisciplinary

Characterization of Giant Modular PKSs Provides Insight into Genetic Mechanism for Structural Diversification of Aminopolyol Polyketides

Lihan Zhang et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2017)

Review Biochemistry & Molecular Biology

Genetic engineering of modular PKSs: from combinatorial biosynthesis to synthetic biology

Kira J. Weissman

NATURAL PRODUCT REPORTS (2016)

Review Biochemistry & Molecular Biology

Biosynthesis of polyketides by trans-AT polyketide synthases

Eric J. N. Helfrich et al.

NATURAL PRODUCT REPORTS (2016)

Article Chemistry, Multidisciplinary

Macrodiolide Formation by the Thioesterase of a Modular Polyketide Synthase

Yongjun Zhou et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2015)

Article Biochemistry & Molecular Biology

Iterative Mechanism of Macrodiolide Formation in the Anticancer Compound Conglobatin

Yongjun Zhou et al.

CHEMISTRY & BIOLOGY (2015)

Article Biochemistry & Molecular Biology

Structural and functional studies of a trans-acyltransferase polyketide assembly line enzyme that catalyzes stereoselective α- and β-ketoreduction

Shawn K. Piasecki et al.

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS (2014)

Article Chemistry, Multidisciplinary

Minimally Invasive Mutagenesis Gives Rise to a Biosynthetic Polyketide Library

Susanna Kushnir et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2012)

Article Chemistry, Multidisciplinary

Functionally Distinct Modules Operate Two Consecutive alpha,beta ->beta,gamma Double-Bond Shifts in the Rhizoxin Polyketide Assembly Line

Bjoern Kusebauch et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2010)

Review Chemistry, Multidisciplinary

The Biosynthetic Logic of Polyketide Diversity

Christian Hertweck

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2009)

Review Biochemistry & Molecular Biology

Isolation, biology and chemistry of the disorazoles: new anti-cancer macrodiolides

Chad D. Hopkins et al.

NATURAL PRODUCT REPORTS (2009)

Article Chemistry, Multidisciplinary

Biosynthesis of the antibiotic bacillaene, the product of a giant polyketide synthase complex of the trans-AT family

Jana Moldenhauer et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2007)

Review Chemistry, Multidisciplinary

Assembly-line enzymology for polyketide and nonribosomal peptide antibiotics: Logic, machinery, and mechanisms

Michael A. Fischbach et al.

CHEMICAL REVIEWS (2006)

Article Chemistry, Multidisciplinary

Total synthesis of (-)-disorazole C1

P Wipf et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2004)